RNA sequencing (RNA-Seq) is the current method of choice for characterizing transcriptomes and
quantifying gene expression changes. This next generation sequencing-based method provides unprecedented
depth and resolution. The negative binomial (NB) probability distribution has been shown to be a
useful model for frequencies of mapped RNA-Seq reads and consequently provides...
We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer...
We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer...
We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer...
We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer...
Many plant-associated Gram-negative bacteria, such a Pseudomonas syringae pv tomato DC3000 (PtoDC3000), use a type III secretion system (T3SS) to establish a host-microbe relationship with compatible plants. The T3SS resembles a molecular injection mechanism that allows the bacteria to deliver type III effectors (T3Es) directly into the cell of the...
Full Text:
. Thireault, Jeff H. Chang
Abstract
Many plant-associated Gram-negative bacteria, such a Pseudomonas
Many plant-associated Gram-negative bacteria, such a Pseudomonas syringae pv tomato DC3000 (PtoDC3000), use a type III secretion system (T3SS) to establish a host-microbe relationship with compatible plants. The T3SS resembles a molecular injection mechanism that allows the bacteria to deliver type III effectors (T3Es) directly into the cell of the...
Full Text:
type III effectors
Andres Alvarez, Allison L. Creason, Caitlin A. Thireault, Jeff H. Chang
Abstract
Many plant-associated Gram-negative bacteria, such a Pseudomonas syringae pv tomato DC3000 (PtoDC3000), use a type III secretion system (T3SS) to establish a host-microbe relationship with compatible plants. The T3SS resembles a molecular injection mechanism that allows the bacteria to deliver type III effectors (T3Es) directly into the cell of the...
36 strains of Burkholderia spp. isolated from sugarcane were evaluated for biological control of leaf and pseudobulb necrosis of orchid caused by B. gladioli. 29 of the sugarcane strains suppressed the disease in greenhouse assays. We generated a draft genomic sequence of one suppressive strain, B. seminalis TC3.4.2R3. The genome...