The advent of high-throughput sequencing (HTS) methods has enabled direct approaches to quantitatively profile small RNA
populations. However, these methods have been limited by several factors, including representational artifacts and lack of
established statistical methods of analysis. Furthermore, massive HTS data sets present new problems related to data processing
and...
In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short
interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target
loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing
response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Eukaryotic small RNA (~20-30 nucleotides) are diverse regulatory molecules that repress gene expression at the transcriptional and post-transcriptional levels, defend hosts against invading viruses and defend genomes against selfish DNA elements. Small RNA populations are studied by high-throughput sequencing of the total small RNA fraction isolated from cells, however, the...