Index Catalog // ScholarsArchive@OSU

Measuring extracellular enzymes in pure and mixed cultures of Trametes versicolor (L.:Fr.) Pilat and Trichoderma harzianum Rifai

Creator:: Freitag, Michael
Abstract:: The biocontrol potential of Trichoderma harzianum Rifai, Trichoderma polysporum (Link.:Pers.) Rifai, Scytalidium aurantiacum Klingstr. et Beyer or a Penicillium sp. against Trametes versicolor (L. :Fr.) Pilat, Neolentinus lepideus (Fr.) Redh. et Ginns, Postia placenta (Fr.) M.Lars. et Lomb. and Irpex lacteus Fr. was evaluated using agar plate, soil bottle and...
Resource Type:: Masters Thesis

The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei

Creator:: Seiboth, Bernhard, Karimi, Razieh Aghcheh, Phatale, Pallavi A., Linke, Rita, Hartl, Lukas, Sauer, Dominik G., Smith, Kristina M., Baker, Scott E., Freitag, Michael, and Kubicek, Christian P.
Abstract:: Trichoderma reesei is an industrial producer of enzymes that degrade lignocellulosic polysaccharides to soluble monomers, which can be fermented to biofuels. Here we show that the expression of genes for lignocellulose degradation are controlled by the orthologous T. reesei protein methyltransferase LAE1. In a lae1 deletion mutant we observed a...
Resource Type:: Article

Heterokaryon Incompatibility Is Suppressed Following Conidial Anastomosis Tube Fusion in a Fungal Plant Pathogen

Creator:: Ishikawa, Francine H., Souza, Elaine A., Shoji, Jun-ya, Connolly, Lanelle, Freitag, Michael, Read, Nick D., and Roca, M. Gabriela
Abstract:: It has been hypothesized that horizontal gene/chromosome transfer and parasexual recombination following hyphal fusion between different strains may contribute to the emergence of wide genetic variability in plant pathogenic and other fungi. However, the significance of vegetative (heterokaryon) incompatibility responses, which commonly result in cell death, in preventing these processes...
Resource Type:: Article

Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence

Creator:: Lepesant, Julie M. J., Cosseau, Celine, Boissier, Jerome, Freitag, Michael, Portela, Julien, Climent, Deborah, Perrin, Cecile, Zerlotini, Adhemar, and Grunau, Christoph
Abstract:: Background: In the leuphotrochozoan parasitic platyhelminth Schistosoma mansoni, male individuals are homogametic (ZZ) whereas females are heterogametic (ZW). To elucidate the mechanisms that led to the emergence of sex chromosomes, we compared the genomic sequence and the chromatin structure of male and female individuals. As for many eukaryotes, the lower...
Resource Type:: Article

Genome resequencing reveals multiscale geographic structure and extensive linkage disequilibrium in the forest tree Populus trichocarpa

Creator:: Slavov, Gancho T., DiFazio, Stephen P., Martin, Joel, Schackwitz, Wendy, Muchero, Wellington, Rodgers-Melnick, Eli, Lipphardt, Mindie F., Pennacchio, Christa P., Hellsten, Uffe, Pennacchio, Len A., Gunter, Lee E., Ranjan, Priya, Vining, Kelly, Pomraning, Kyle R., Wilhelm, Larry J., Pellegrini, Matteo, Mockler, Todd C., Freitag, Michael, Geraldes, Armando, El-Kassaby, Yousry A., Mansfield, Shawn D., Cronk, Quentin C. B., Douglas, Carl J., Strauss, Steven H., Rokhsar, Dan, and Tuskan, Gerald A.
Abstract:: •Plant population genomics informs evolutionary biology, breeding, conservation and bioenergy feedstock development. For example, the detection of reliable phenotype–genotype associations and molecular signatures of selection requires a detailed knowledge about genome-wide patterns of allele frequency variation, linkage disequilibrium and recombination. •We resequenced 16 genomes of the model tree Populus trichocarpa...
Resource Type:: Article

Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression

Creator:: Vining, Kelly J., Pomraning, Kyle R., Wilhelm, Larry J., Priest, Henry D., Pellegrini, Matteo, Mockler, Todd C., Freitag, Michael, and Strauss, Steven H.
Abstract:: Background: DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylation among specialized tissues and its relationship to gene expression is poorly understood. Results: We surveyed DNA methylation from...
Resource Type:: Article

Comparative Genomics of a Plant-Pathogenic Fungus, Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Creator:: Manning, Viola A., Pandelova, Iovanna, Dhillon, Braham, Wilhelm, Larry J., Goodwin, Stephen B., Berlin, Aaron M., Figueroa, Melania, Freitag, Michael, Hane, James K., Henrissat, Bernard, Holman, Wade H., Kodira, Chinnappa D., Martin, Joel, Oliver, Richard P., Robbertse, Barbara, Schackwitz, Wendy, Schwartz, David C., Spatafora, Joseph W., Turgeon, B. Gillian, Yandava, Chandri, Young, Sarah, Zhou, Shiguo, Zeng, Qiandong, Grigoriev, Igor V., Ma, Li-Jun, and Ciuffetti, Lynda M.
Abstract:: Pyrenophora tritici-repentis is a necrotrophic fungus causal to the disease tan spot of wheat, whose contribution to crop loss has increased significantly during the last few decades. Pathogenicity by this fungus is attributed to the production of host-selective toxins ( HST), which are recognized by their host in a genotype-specific...
Resource Type:: Article

Bacterial Resistance to Antisense Peptide-Phosphorodiamidate Morpholino Oligomers

Creator:: Puckett, Susan E., Reese, Kaleb A., Mitev, Georgi M., Mullen, Valerie, Johnson, Rudd C., Pomraning, Kyle R., Mellbye, Brett L., Tilley, Lucas D., Iversen, Patrick L., Freitag, Michael, and Geller, Bruce L.
Abstract:: Peptide phosphorodiamidate morpholino oligomers (PPMO) are synthetic DNA mimics that bind complementary RNA and inhibit bacterial gene expression. (RFF)₃RXB- AcpP PPMO (R, arginine; F, phenylalanine; X, 6-aminohexanoic acid; B, β-alanine) is complementary to 11 bases of the essential gene acpP (encodes acyl carrier protein). The MIC of (RFF)₃RXB-AcpP was 2.5...
Resource Type:: Article

Functional Analyses of Trichoderma reesei LAE1 Reveal Conserved and Contrasting Roles of This Regulator

Creator:: Karimi-Aghcheh, Razieh, Bok, Jin Woo, Phatale, Pallavi A., Smith, Kristina M., Baker, Scott E., Lichius, Alexander, Omann, Markus, Zeilinger, Susanne, Seiboth, Bernhard, Rhee, Catherine, Keller, Nancy P., Freitag, Michael, and Kubicek, Christian P.
Abstract:: The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi, and it can modify heterochromatin structure in Aspergillus nidulans. We have recently shown that the LaeA ortholog of Trichoderma reesei (LAE1), a fungus that is an industrial producer of...
Resource Type:: Article

Identification of the Polycomb Repressive Complex 2 in Fungi

Creator:: Pham, Phuong and Freitag, Michael
Abstract:: The process of expressing a gene involves not just binding of proteins and enzymes to DNA to produce first RNA and eventually proteins, but rather association of these transcription factors with chromatin and structural proteins called histones. When histone H3 is trimethylated at lysine 27 to produce H3K27me3, gene silencing...
Resource Type:: Honors College Thesis

ScholarsArchive@OSU

Measuring extracellular enzymes in pure and mixed cultures of Trametes versicolor (L.:Fr.) Pilat and Trichoderma harzianum Rifai

The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei

Heterokaryon Incompatibility Is Suppressed Following Conidial Anastomosis Tube Fusion in a Fungal Plant Pathogen

Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence

Genome resequencing reveals multiscale geographic structure and extensive linkage disequilibrium in the forest tree Populus trichocarpa

Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression

Comparative Genomics of a Plant-Pathogenic Fungus, Pyrenophora tritici-repentis, Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence

Bacterial Resistance to Antisense Peptide-Phosphorodiamidate Morpholino Oligomers

Functional Analyses of Trichoderma reesei LAE1 Reveal Conserved and Contrasting Roles of This Regulator

Identification of the Polycomb Repressive Complex 2 in Fungi

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