Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts...
Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts...
Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts...
Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts...
In response to genotoxic stress, ATR and ATM kinases phosphorylate H2A in fungi and H2AX in animals on a C-terminal serine.
The resulting modified histone, called γH2A, recruits chromatin-binding proteins that stabilize stalled replication forks or promote
DNA double-strand-break repair. To identify genomic loci that might be prone to replication...
In response to genotoxic stress, ATR and ATM kinases phosphorylate H2A in fungi and H2AX in animals on a C-terminal serine.
The resulting modified histone, called γH2A, recruits chromatin-binding proteins that stabilize stalled replication forks or promote
DNA double-strand-break repair. To identify genomic loci that might be prone to replication...
The process of expressing a gene involves not just binding of proteins and enzymes to DNA to produce first RNA and eventually proteins, but rather association of these transcription factors with chromatin and structural proteins called histones. When histone H3 is trimethylated at lysine 27 to produce H3K27me3, gene silencing...
The process of expressing a gene involves not just binding of proteins and enzymes to DNA to produce first RNA and eventually proteins, but rather association of these transcription factors with chromatin and structural proteins called histones. When histone H3 is trimethylated at lysine 27 to produce H3K27me3, gene silencing...
Fungal hyphae are among the most highly polarized cells. Hyphal polarized
growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily
in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex
is required for tethering of secretory vesicles to the apical plasma membrane. We...
Fungal hyphae are among the most highly polarized cells. Hyphal polarized
growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily
in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex
is required for tethering of secretory vesicles to the apical plasma membrane. We...