Here we report a new transgenic expression system by combination of liver-specific expression, mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure of mifepristone to induce permanent genomic recombination mediated by...
Here we report a new transgenic expression system by combination of liver-specific expression,
mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic
kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure
of mifepristone to induce permanent genomic recombination mediated by...
Full Text:
expression in transgenic zebrafish
Anh Tuan Nguyen
1, 2
, Vivien Koh
1
, Jan M. Spitsbergen
2
Here we report a new transgenic expression system by combination of liver-specific expression,
mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic
kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure
of mifepristone to induce permanent genomic recombination mediated by...
Full Text:
Anh Tuan Nguyen
Vivien Koh
Jan M. Spitsbergen
Zhiyuan
Hepatocellular carcinoma (HCC) is currently one of the top lethal cancers with an increasing
trend. Deregulation of MYC in HCC is frequently detected and always correlated with poor
prognosis. As the zebrafish genome contains two differentially expressed zebrafish myc
orthologs, myca and mycb, it remains unclear about the oncogenicity of...
Full Text:
Mutation
Sun, L., Nguyen, A. T., Spitsbergen, J. M., & Gong, Z. (2015). Myc-Induced Liver
Tumors in
Hepatocellular carcinoma (HCC) is currently one of the top lethal cancers with an increasing
trend. Deregulation of MYC in HCC is frequently detected and always correlated with poor
prognosis. As the zebrafish genome contains two differentially expressed zebrafish myc
orthologs, myca and mycb, it remains unclear about the oncogenicity of...
Hepatocellular carcinoma (HCC) is currently one of the top lethal cancers with an increasing trend. Deregulation of MYC in HCC is frequently detected and always correlated with poor prognosis. As the zebrafish genome contains two differentially expressed zebrafish myc orthologs, myca and mycb, it remains unclear about the oncogenicity of...
Because Ras signaling is frequently activated by major hepatocellular carcinoma etiological factors, a transgenic zebrafish constitutively expressing the kras(V12) oncogene in the liver was previously generated by our laboratory. Although this model depicted and uncovered the conservation between zebrafish and human liver tumorigenesis, the low tumor incidence and early mortality...
Human liver cancer is one of the deadliest cancers worldwide, with hepatocellular carcinoma (HCC) being the most common type. Aberrant Ras signaling has been implicated in the development and progression of human HCC, but a complete understanding of the molecular mechanisms of this protein in hepatocarcinogenesis remains elusive. In this...
RAS and Rho small GTPases are key molecular switches that control cell dynamics, cell
growth and tissue development through their distinct signaling pathways. While much has been
learnt about their individual functions in both cell and animal models, the physiological and
pathophysiological consequences of their signaling crosstalk in multi-cellular context...