Index Catalog // ScholarsArchive@OSU

Creator:: Tang, Longteng, Zhu, Liangdong, Wang, Yanli, and Fang, Chong
Abstract:: Intracellular pH (pH(i)) imaging is of paramount importance for life sciences. In this work, we implement the ultrafast electronic and stimulated Raman spectroscopies to unravel the fluorescence mechanism of an excitation-ratiometric pH(i) indicator in basic aqueous solution. After photoexcitation of the pH(i) indicator HPTS, a hidden charge-transfer (CT) state following...
Resource Type:: Article
Full Text:: of more efficient and bright pHi indicators in an H-bonding environment with controllable

Creator:: Han, Fangyuan, Liu, Weimin, and Fang, Chong
Abstract:: We use femtosecond stimulated Raman spectroscopy (FSRS) to illuminate the choreography of intermolecular excited-state proton transfer (ESPT) of photoacid pyranine (8-hydroxypyrene-1,3,6-trisulfonic acid, HPTS) in water. The multidimensional reaction coordinate responsible for photoacidity is revealed to involve sequential activation of characteristic skeletal motions during the ca. 1 ps preparation stage preceding...
Resource Type:: Article
Full Text:: (108 cm -1 ) and ring-H out-of-plane motions (321, 362, 952 cm -1 ), which largely decay within ~1

Creator:: Tachibana, Sean R., Tang, Longteng, Zhu, Liangdong, Liu, Weimin, Wang, Yanli, and Fang, Chong
Abstract:: Biosensors have become an indispensable tool set in life sciences. Among them, fluorescent protein-based biosensors have great biocompatibility and tunable emission properties but their development is largely on trial and error. To facilitate a rational design, we implement tunable femtosecond stimulated Raman spectroscopy, aided by transient absorption and quantum calculations,...
Resource Type:: Article
Full Text:: , less compact, and more homogeneous environment with directional H- bonding (from the chromophore to

Creator:: Wang, Yanli, Tang, Longteng, Liu, Weimin, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Resource Type:: Article
Full Text:: in the context of Franck-Condon (FC) proton motions via pre-existing H-bonding chains. The data

Creator:: Tang, Longteng, Liu, Weimin, Wang, Yanli, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Imaging Ca²⁺ dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca²⁺-biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca²⁺ binding, a significant population of the chromophores becomes deprotonated. It...
Resource Type:: Article
Full Text:: imidazolinone ring deformation and C–H rocking) redshifts to 1037 cm-1 with a marked intensity drop, while the

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