This study presents a methods evaluation and intercalibration of active fluorescence-based measurements of the quantum yield (ϕ'ₚₛᵢᵢ) and absorption coefficient (aₚₛᵢᵢ) of photosystem II (PSII) photochemistry. Measurements of ϕ'ₚₛᵢᵢ, aₚₛᵢᵢ, and irradiance (E) can be scaled to derive photosynthetic electron transport rates (Pₑ), the process that fuels phytoplankton carbon fixation...