The spectrophotometric titration of the tyrosine residues,
their reaction with N-acetylimidazole, and the effect of pH on the
thermally induced conformational transition of ribonuclease T₁ have
been investigated. The results of spectrophotometric titration in
0.20 M KCl/0.001 M sodium phosphate suggest that titration of the
tyrosine residues occurs in two...
Published June 1992. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog