Proteinaceous compounds are abundant forms of organic nitrogen in soil and aquatic ecosystems, and the rate of protein depolymerization, which is accomplished by a diverse range of microbial secreted peptidases, often limits nitrogen turnover in the environment. To determine if the distribution of secreted peptidases reflects the ecological and evolutionary...
Research in warm-climate biomes has shown
that invasion by symbiotic dinitrogen (N₂)-fixing plants can
transform ecosystems in ways analogous to the transformations
observed as a consequence of anthropogenic, atmospheric
nitrogen (N) deposition: declines in biodiversity, soil
acidification, and alterations to carbon and nutrient cycling,
including increased N losses through nitrate...
Research in warm-climate biomes has shown
that invasion by symbiotic dinitrogen (N₂)-fixing plants can
transform ecosystems in ways analogous to the transformations
observed as a consequence of anthropogenic, atmospheric
nitrogen (N) deposition: declines in biodiversity, soil
acidification, and alterations to carbon and nutrient cycling,
including increased N losses through nitrate...
A new functional gene database, FOAM (Functional
Ontology Assignments for Metagenomes), was developed
to screen environmental metagenomic sequence
datasets. FOAM provides a new functional
ontology dedicated to classify gene functions relevant
to environmental microorganisms based on Hidden
Markov Models (HMMs). Sets of aligned protein
sequences (i.e. ‘profiles’) were tailored to...
A new functional gene database, FOAM (Functional
Ontology Assignments for Metagenomes), was developed
to screen environmental metagenomic sequence
datasets. FOAM provides a new functional
ontology dedicated to classify gene functions relevant
to environmental microorganisms based on Hidden
Markov Models (HMMs). Sets of aligned protein
sequences (i.e. ‘profiles’) were tailored to...
Although ammonia-oxidizing archaea (AOA) and bacteria (AOB) coexist in most non-acidic agricultural soils, the factors that influence their relative contributions to soil nitrification activity remain unclear. A 2- to 4-d whole soil microcosm assay was developed, utilizing the aliphatic C₈ alkyne 1-octyne to inactivate AOB-driven nitrification activity without impacting AOA...
A new functional gene database, FOAM (Functional Ontology Assignments for Metagenomes), was developed to screen environmental metagenomic sequence datasets. FOAM provides a new functional ontology dedicated to classify gene functions relevant to environmental microorganisms based on Hidden Markov Models (HMMs). Sets of aligned protein sequences (i.e. ‘profiles’) were tailored to...
Research in warm-climate biomes has shown that invasion by symbiotic dinitrogen (N₂)-fixing plants can transform ecosystems in ways analogous to the transformations observed as a consequence of anthropogenic, atmospheric nitrogen (N) deposition: declines in biodiversity, soil acidification, and alterations to carbon and nutrient cycling, including increased N losses through nitrate...
It is well known that the ratio of ammonia oxidizing archaea (AOA) and bacteria (AOB) ranges widely in soils, but no data exist on what might influence this ratio, its dynamism, or how changes in relative abundance influences the potential contributions of AOA and AOB to soil nitrification. By sampling...
Ammonia (NH₃)-oxidizing bacteria (AOB) and thaumarchaea (AOA) co-occupy most soils, yet no short-term growth-independent method exists to determine their relative contributions to nitrification in situ. Microbial monooxygenases differ in their vulnerability to inactivation by aliphatic n-alkynes, and we found that NH₃ oxidation by the marine thaumarchaeon Nitrosopumilus maritimus was unaffected...