Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and thawed sperm: Blaxter (1953); Hoyle and Idler (1968); and Graybill and Horton (1969). Significant advances reported in this thesis were: the development of suitable life...
Successful fertilization of fresh steelhead trout, pink salmon, kokanee, chum salmon, fall chinook salmon, and coho salmon eggs with cryopreserved sperm is reported in this thesis. A mean percent fertilization greater than 50 percent was achieved for all species tested except chum salmon. Differences in sperm viability from donor males...
