Fecal pollution is measured in surface waters using culture-based measurements of
enterococci and Escherichia coli bacteria. Source apportionment of these two fecal indicator
bacteria is an urgent need for prioritizing remediation efforts and quantifying health risks
associated with source-specific pathogens. There are a number of quantitative real-time
PCR (QPCR) assays...
Fecal pollution is measured in surface waters using culture-based measurements of
enterococci and Escherichia coli bacteria. Source apportionment of these two fecal indicator
bacteria is an urgent need for prioritizing remediation efforts and quantifying health risks
associated with source-specific pathogens. There are a number of quantitative real-time
PCR (QPCR) assays...
There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality is typically determined through a series...
Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely...
Fecal pollution is measured in surface waters using culture-based measurements of enterococci and Escherichia coli bacteria. Source apportionment of these two fecal indicator bacteria is an urgent need for prioritizing remediation efforts and quantifying health risks associated with source-specific pathogens. There are a number of quantitative real-time PCR (QPCR) assays...
Genetic markers from Bacteroides and other faecal bacteria are being tested for inclusion in regulations to quantify aquatic faecal contamination and estimate public health risk. For the method to be used quantitatively across environments, persistence and decay of markers must be understood. We measured concentrations of contaminant molecular markers targeting...
The studies described here introduce a model for residue preservation on stone tools. They simulate stone tool manufacture in order to define parameters important for the study of DNA residues. Microscopic examination of stone tools has identified microcracks that trap DNA and protein from animal blood cells. Thorough investigation of...