Degradation and release to solution of intracellular dimethylsulfoniopropionate (DMSP) from Emiliania huxleyi 370 was observed during grazing by the heterotrophic dinoflagellate Oxyrrhis marina in 24 h bottle incubations. Between 30 and 70 % of the lost algal DMSP was metabolized by the grazers without production of dimethylsulfide (DMS) when grazer...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net tow stations along the Newport Hydro line, to make continuous bio-acoustic observations between the 50-500m. isobath, and to make continuous observations of...
Previous work has demonstrated selective feeding by bacterivorous protists on the of prey size. Other prey characteristics may also affect protist grazing rates. In this relative uptake rates of fluorescently labeled bacterial (FLB) cells made from starving bacteria (S-FLB), and of live-stained motile vs non-motile bacteria by marine We also...
Three staining methods were used to identify metabolically active bacteria in Lake Kinneret, northern Israel: CTC, DAPI staining followed by a propanol wash, and the Molecular Probes Live/Dead stain. Positive results from these methods purport to show, respectively, actively respiring bacteria (CTC+), cells with intact nucleoids (NuCC), and cells with...
A new planktonic ecosystem model was constructed for the Eastern Bering Sea based on observations from the 2007–2010 BEST/BSIERP (Bering Ecosystem Study/Bering Sea Integrated Ecosystem Research Program) field program. When run with forcing from a data-assimilative ice-ocean hindcast of 1971–2012, the model performs well against observations of spring bloom time...
We evaluated the extent to which the average metabolic activity of open ocean bacterioplankton
changed during 2 to 3 d incubations of 1 μm filtered seawater at in situ temperature. Indices
of bacterial activity during incubation experiments were compared to those of in situ bacterioplankton,
which were repeatedly sampled at...
A variety of macromolecules labeled with FITC (fluorescein isothiocyanate), including
carbohydrates (dextran) and proteins (ferritin, casein, albumin, concavalin A), spanning a range of
molecular weights from 55 to 2000 kD, were actively ingested by marine heterotrophic flagellates at
substrate concentrations of 1 to 10 mg/L. The uptake capacity for the...
Grazing by phagotrophic flagellates and ciliates is a major source of mortality for bacterioplankton in both
marine and freshwater systems. Recent studies have demonstrated a positive relationship between clearance
rate and prey size for bacterivorous protists. We tested the idea that, by selectively grazing the larger (more
actively growing or...
We propose quantification of the activity of digestive enzymes as a novel way to estimate
rates of protist grazing in natural waters. Our first application of this approach was determination of
protistan bacterivory by assaying the activity of lysozyme at acid pH. Lysozyme specifically degrades
peptidoglycan, a major structural component...
The redox dye 5-cyano-2, 3-ditolyl tetrazolium chloride (CTC) is used in aquatic sciences as a vital stain for enumeration of respiring bacteria in situ. Questions concerning its efficacy have been raised. We propose that the abundance of CTC-positive (CTC+) bacteria is a useful parameter in microbial ecology based on the...
Protists are microscopic eukaryotic
microbes that are ubiquitous, diverse,
and major participants in oceanic food
webs and in marine biogeochemical
cycles. The study and characterization
of protists has a long and distinguished
tradition. Even with this history, the
extraordinary species diversity and variety
of interactions of protists in the sea...
Recent data on seasonal variation in the concentration of atmospheric oxygen are independent evidence for a marked annual cycle in the production/respiration (P/R) ratio of the biotic community of the ocean in both the northern and southern hemispheres. Based on the oxygen data, the P/R ratio tends to be >1...
We evaluated whether bacteria with higher cell-specific nucleic acid content (HNA) or an active electron transport system, i.e., positive for reduction of 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), were responsible for the bulk of bacterioplankton metabolic activity. We also examined whether the phylogenetic diversity of HNA and CTC-positive cells differed from the...
Variation in bacterial abundance and activity was assessed by sampling the upper 35 to 80 m of the water column during 2 to 5 d periods at 3 sites: eutrophic-mesotrophic midshelf, mesotrophic-oligotrophic slope, and oligotrophic gyre edge, off the Oregon coast in late summer 1997 and 1998. Bacterial abundances varied...
Small, aloricate ciliates with equivalent spherical cell diameters <20 µm (biovolume <4,000 µm3) compose, on average, 4–57% of the total biomass of heterotrophic (apochlorotic) nanoplankton in diverse marine systems. Biomass production of nanoplanktonic ciliates in a southeastern U.S. estuary was also a significant part of the total production of heterotrophic...
We compared estimates of bacterial abundance and of average bacterial cell size based on direct counts of bacterioplankton cells stained with acridine orange (AO) or 4’-6-diamidino-2-phenylidole (DAPI). Estimates of numbers mll–1 of bacteria stained with DAPI were on average 70% (range 57–82%) of bacterial counts made with AO as the...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to make continuous...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net tow stations along the Newport Hydro line, to make continuous bio-acoustic observations between the 50-500m. isobath, and to make continuous observations of...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to make continuous...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in the NE Pacific. In particular, to make CTD/rosette and
net tow stations along the Newport Hydro line, to make continuous bio-acoustic observations
between the 50-500m. isobath, and to make continuous observations of currents...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to make continuous...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to make continuous...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to make continuous...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along the Newport Hydro line, and to make continuous observations of currents using
ADCP and of surface-layer temperature, salinity and...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along the Newport Hydro line, and to make continuous observations of currents using
ADCP and of surface-layer temperature, salinity and...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, the Rogue River, OR. and Crescent
City, CA.), to deploy drifters...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 5 lines (off Newport, Heceta Head, Coos Bay, OR. and Crescent City, CA.), to
deploy drifters at selected locations...
PURPOSE: To determine physical, plankton and nutrient/chemical conditions over the continental
margin for climate change studies in NE Pacific. In particular, to make CTD and CTD/rosette and net
tow stations along 4 lines (off Newport, Heceta Head, Coos Bay, OR. and Crescent City, CA.), to
deploy drifters at selected locations...