Desferrioxamine B (DFOB) was reversibly and covalently conjugated to polystyrene microspheres via N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) crosslinker. DFOB retained post-immobilization activity with at least 89% able to bind Fe(III)-citrate under pH 7.4 and a citrate:iron molar ratio = 100:1. Comparing iron capture by DFOB-activated beads and sulfhydryl (−SH) control beads demonstrated...
The immobilization of chelators to solid supports has recently gained renewed interest due to the increasing biomedical and environmental applications made possible by these molecules’ ability to bind Fe(III) and other metal ions. The uses of immobilized chelators have expanded to include the treatment of metal overload in serum, the...