The use of radioactive isotopes as tracers in biological systems
has become widespread since the close of World War II. Proper
use of radiotracers requires a fundamental understanding of the physical
nature of radioactivity, the characteristics of ionizing radiation,
and the various methods available for measuring radioactivity.
More importantly, the...
Some kinetics of the oxygen requirement of amine oxidases
have been investigated using bovine plasma monoamine oxidase (PAO)
and pea seedling diamine oxidase (DAO). A system is described for
studying enzyme reactions under controlled oxygen tension and in an
O₂-free N₂ atmosphere.
O₂ Km values were determined at two different...