Contracting nucleolar vacuoles are a general and consistent
feature of somatic tobacco cells growing in microcultures. Nucleolar
vacuoles were studied in cells from callus tissues of two
single-cell clones, H-196 and H-239, of hybrid tobacco (Nicotiana
tabacum X N. glutinosa). Vacuoles periodically contracted either
completely or incompletely, apparently releasing a...
A cell-free system for assessing the role of S-adenosyl-methionine
in the methylation of homocysteine deriving the methyl
group from serine has been developed. No role for S-adenosyl-methionine
could be shown in the in vitro reaction
The enzyme serine transhydroxymethylase has been studied in
crude and partially purified cell extracts. Evidence...
There are numerous references in the literature concerning
the use of various Arctostaphylos species as medicinal plants.
One of the species, A. uva-ursi, was listed in the United States
official compendia from 1820 until 1946 as a urinary antiseptic.
Many species indigenous to the Pacific Northwest were employed
by the...
An enzyme system was prepared from Saccharomyces cerevisiae,
strain MCC, which carries out the transfer of the methyl group from
S-adenosylmethionine or methionine to the side chain of yeast sterols.
The cell-free system has been shown to be incapable of synthesizing
ergosterol but carries out the synthesis of two as...
Ribonucleotide reductase from Rhizobium meliloti has been
partially purified and characterized. The enzymatic reduction of
ribonucleotides to deoxyribonucleotides is dependent upon B₁₂ coenzyme
and dihydrolipoate.
B₁₂ coenzyme was more effective than B₁₂ coenzyme analogues
in the reductase reaction. The affinity of the R. meliloti
reductase system for B₁₂ coenzyme was...