The increasing knowledge of the significance of vitamin B₆ in
human metabolic processes has emphasized the importance of developing
reliable means of assessing nutritional status with respect to
this vitamin. One possible approach to this end is the measurement
of pyridoxal phosphate in blood or plasma, particularly since this
phosphorylated...
4-Pyridoxic acid is the major metabolite of all forms of
vitamin B₆ in mammalian species. The determination of 4-pyridoxic
acid, therefore, is important in the investigation of vitamin B₆
metabolism and for the establishment of human requirements for the
vitamin.
Although 4-pyridoxic acid is itself fluorescent, Huff and
Perlzweig (1944)...
Pyridoxal phosphate is a coenzyme in about 50 known enzymatic
reactions. A simple and accurate method for the determination of
pyridoxal phosphate would be desirable because it could provide a
means to assess the nutritional status of vitamin B₆ in the human.
The cyanohydrin methods to determine pyridoxal phosphate appear...