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  • Improved coagulase tests for Staphylococcus aureus

    Creator:
    Orth, Donald Simms
    Abstract:
    The need for better procedures for detecting Staphylococcus aureus prompted an investigation to improve coagulase testing. The rates of coagulase clotting were studied by using varying concentrations of trypsin inhibitor and coagulase-reacting factor (CRF). Egg white trypsin inhibitor activated coagulase clotting when added to a final concentration between 2 and...
    Resource Type:
    Dissertation
    Full Text:
    Imrpoved coagulase tests for Staphylococcus aureus AN

  • Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus

    Creator:
    Chugg, Lee Russon
    Abstract:
    A selective, differential plating medium for the enumeration of coagulase-positive staphylococci was developed. The ingredients of this medium were analysed for their effect on normal, heat treated and frozen staphylococcal cells. The nutrient base consisted of 10 gm Tryptone, 5 gm Beef Extract, 1 gm Yeast Extract and 17 gm...
    Resource Type:
    Dissertation
    Full Text:
    assay for Staphylococcus aureus AN ABSTRACT OF THE THESIS OF LEE RUSSON CHUGG for the DOCTOR OF

  • Serological detection of staphylococcal enterotoxin A in culture supernatants

    Creator:
    Nkanga, Enefiok James
    Abstract:
    A method has been presented for the observation of a large number of enterotoxin A producers. Production of a relatively concentrated toxin is achieved by the sac culture method. Further concentration, if needed, is carried out by PM-10 membrane ultrafiltration after centrifugation. The work is further improved by trypsinization of...
    Resource Type:
    Masters Thesis
    Full Text:
    human hands, nose, face, respiratory and gastrointestinal tract. Staphylococcus epidermidis is the

  • Growth, enterotoxin production and energy charge of Staphylococcus aureus under three gaseous environments

    Creator:
    Park, Sue Sookja
    Abstract:
    The effect of different oxygen atmospheres on growth, energy charge and enterotoxin production of Staphylococcus aureus was investigated. Three atmospheres, air, 5% oxygen in nitrogen and 20% oxygen in nitrogen, were tested on three strains producing enterotoxins A, B and C. Colony forming units (CFU), dry weight, dissolved oxygen, pH,...
    Resource Type:
    Dissertation
    Full Text:
    Department) (Date) Title: GROWTH, ENTEROTOXIN PRODUCTION AND ENERGY CHARGE OF STAPHYLOCOCCUS AUREUS

  • Growth and enterotoxin production of Staphylococcus aureus in papain-treated beef and ham

    Creator:
    Zipperer, Sharon Ann
    Abstract:
    Growth and enterotoxin production of Staphylococcus aureus in papain-treated beef and ham were studied. In addition to an untreated control, four treatments were used in the beef experiment including two levels of papain, a commercial tenderizer preparation, and commercially tenderized beef. Two levels of papain treatment and an untreated control...
    Resource Type:
    Masters Thesis
    Full Text:
    / 7 /^* (Major) NPate') Title: GROWTH.AND ENTEROTOXIN PRODUCTION OF STAPHYLOCOCCUS AUREUS IN