The lack of a suitable demembranated cell model has hindered the
study of the mechanisms responsible for the regulation of ciliary
waveform and coordination. In this study procedures for the
isolation of ciliated cells from the newt, Taricha granulosa, by
trypsin dissociation, and their subsequent demembranation with Triton
X-100 and...
Sunflower (Helianthus annuus L., cv. NK894) seedlings, grown hydroponically, were exposed to ozone concentrations of 0.0, 0.1, 0.2, and 0.3 ppm at 60% RH, or 0.0
and 0.3 ppm at 30% and 90% RH, for 6 h/d, for four consecutive days, at ages 15 to 18 days from seeding. Plant...
Apoplastic distribution patterns of atrazine [2-chloro-4-(ethylamino)-
6-(isopropylamino)-s-triazine], glyphosate [N-(phosphonomethyl)-
glycine], the naturally occurring apoplastic leaf solutes, and
the apoplastic dye PTS (trisodium 3-hydroxy-5,8,10-pyrenetrisulfonate)
were compared. Aliquots of sap were expressed from intact
sunflower leaves in a pressure chamber over intervals of 0.2 to 0.4
bars. Three distinct fractions were detected...
During 2 seasons effects of high and low irrigation (water applied
at -0.6 and -2.5 bars soil water potential, respectively) and
density were evaluated on yield parameters and flower/pod development
at mainstem nodes 2 and 6 (terminal) of 'Oregon 1604', a determinate
snap bean. High and low plant densities were...
A survey of major bean (Phaseolus vulgaris) breeding programs and
seed producing areas in Idaho, Washington, and Oregon was conducted to
determine the occurrence of cucumber mosaic virus (CMV), recently reported
to be seedborne in beans. Some 259 plants, sampled from breeding
lines of diverse germplasm, were found by enzyme-linked...
A method for isolating the salt gland (SG) from the larval brine
shrimp has been developed. This extra-renal organ is removed from 5-10%
of Stage Inauplii by vortexing after incubating the animals at 37°C for
8 hours in a saline solution buffered with 100 mM sodium phosphate at pH
7.6....