Turnip yellow mosaic virus is a monopartite, plus sense RNA virus
infecting the Cruciferae, and is a model system for the study of RNA virus
replication. A cDNA clone (pTYMC) representing an infectious RNA
genome of the European isolate of TYMV was constructed and used to assess
the importance of...
Vaccinia virus infected monkey kidney cells had been previously shown
to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA
from mutant virus resistant to hydroxyurea were digested with restriction
endonucleases and were shown to have substoichiometric amounts of the Hind
III F fragment. Additional information from Southern blotting experiments...
The complete genome of snakehead rhabdovirus (SHRV) was cloned and
molecularly characterized. This was initially accomplished through the sequence
determination of its glycoprotein gene and the phylogenetic analysis of this gene with
orthologous genes from other rhabdoviruses. The phylogenetic analysis revealed that
SHRV groups with viruses of the genus Novirhabdovirus....
Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting
step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project
was to characterize the ribonucleotide reductase encoded by the orthopoxvirus,
vaccinia. The first objective was to study the structural and functional features of the
viral small subunit...
Thymidine kinase is a key enzyme in the nucleotide salvage pathway,
catalyzing the production of dTMP from thymidine and ATP. In order to
identify the structural features of the TK protein and/or primary amino
acid sequences which contribute to the catalytic and regulatory activities of
this enzyme, an in vitro...
Pea seed-borne mosaic potyvirus pathotype 1 (PSbMV-P1) has the ability to infect most genotypes of Pisum sativum. The exception are those genotypes that are homozygous recessive for the sbm-1 gene. The life cycle of PSbMV pathotype 4 (PSbMV-P4) is unaffected by the sbm-1/sbm-1 genotype. Infectious clones of Pl-P4 recombinants were...