Index Catalog // ScholarsArchive@OSU

Osmotic Tolerance Limits of Red Blood Cells from Umbilical Cord Blood

Creator:: Zhurova, Mariia, Lusianti, Ratih E., Higgins, Adam Z., and Acker, Jason P.
Abstract:: Effective methods for long-term preservation of cord red blood cells (RBCs) are needed to ensure a readily available supply of RBCs to treat fetal and neonatal anemia. Cryopreservation is a potential long-term storage strategy for maintaining the quality of cord RBCs for the use in intrauterine and neonatal transfusion. However,...
Resource Type:: Article
Full Text:: umbilical cord blood. Cryobiology, 69(1), 48-54. doi:10.1016/j.cryobiol.2014.05.001 10.1016/j.cryobiol

Mathematical optimization of procedures for cryoprotectant equilibration using a toxicity cost function

Creator:: Benson, James D., Kearsley, Anthony J., and Higgins, Adam Z.
Abstract:: Cryopreservation nearly universally depends on the equilibration of cells and tissues with high concentrations of permeating chemicals known as cryoprotective agents, or CPAs. Despite their protective properties, CPAs can cause damage as a result of osmotically-driven cell volume changes, as well as chemical toxicity. In this study, we have used...
Resource Type:: Article
Full Text:: Sciences, Dekalb, IL 60115-2888, USA. Cryobiology 64 (2012) 144–151 Contents lists available at SciVerse

The Effect of Human Serum Albumin and Hematocrit on the Cake Collapse Temperature of Lyophilized Red Blood Cells

Creator:: Runyon, Daniel E. and Higgins, Adam Z.
Abstract:: Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s ability to form a stable...
Resource Type:: Article
Full Text:: . Loading red blood cells with trehalose: a step towards biopreservation. Cryobiology 2004;49: 123-136

Measurement of Cryoprotectant Permeability in Adherent Endothelial Cells and Applications to Cryopreservation

Creator:: Fry, Allyson K. and Higgins, Adam Z.
Abstract:: Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...
Resource Type:: Article
Full Text:: . Petrenko, and L. E. McGann. Intracellular ice formation is affected by cell interactions. Cryobiology 38

Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol

Creator:: Vian, Alex M. and Higgins, Adam Z.
Abstract:: Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to...
Resource Type:: Article
Full Text:: of the granules, Cryobiology 33 (1996) 391-403. [6] D. Atay, G. Ozturk, A. Akcay, M. Yanasik, S

Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells

Creator:: Fry Davidson, Allyson
Abstract:: Cryopreservation of adherent cells may be advantageous for cell types that are difficult to preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...
Resource Type:: Dissertation
Full Text:: governing principle of cryobiology that describes the relationship between the rate of temperature change

Membrane permeability properties of human granulocytes

Creator:: Vian, Alexander M.
Abstract:: In the last decade, there has been renewed interest in the use of granulocyte transfusions to treat infections in individuals with compromised immune systems. However, granulocytes only remain functional for about a day after isolation and this short shelf life is a significant drawback. Cryopreservation would allow long term storage...
Resource Type:: Masters Thesis

Optimization of Cryoprotectant Loading into Murine and Human Oocytes

Creator:: Karlsson, Jens O. M., Szurek, Edyta A., Higgins, Adam Z., Lee, Sang R., and Eroglu, Ali
Abstract:: Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity. Thus, we investigated the use of physics-based mathematical optimization to guide design of cryoprotectant loading methods for mouse and human oocytes. We first...
Resource Type:: Article
Full Text:: into murine and human oocytes. Cryobiology, 68(1), 18-28. doi:10.1016/j.cryobiol.2013.11.002 10.1016

Investigating the Potential for Cryopreservation of Human Granulocytes with Concentrated Glycerol

Creator:: Moss, Alden C.
Abstract:: Granulocytes are a class of white blood cells essential to immune function. Granulocyte transfusions hold promise to treat many conditions, but the short-shelf life of granulocytes makes transfusions infeasible without a cell preservation method. The purpose of this study was to investigate the potential for cryopreservation of granulocytes using 30%...
Resource Type:: Honors College Thesis
Full Text:: and ethylene glycol, Cryobiology 68 (2014) 35e42. [5] D.E. Pegg, Principles of Cryopreservation

Mathematically optimized cryoprotectant equilibration procedures for cryopreservation of human oocytes

Creator:: Davidson, Allyson Fry, Benson, James D., and Higgins, Adam Z.
Abstract:: BACKGROUND: Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid...
Resource Type:: Article

ScholarsArchive@OSU

Osmotic Tolerance Limits of Red Blood Cells from Umbilical Cord Blood

Mathematical optimization of procedures for cryoprotectant equilibration using a toxicity cost function

The Effect of Human Serum Albumin and Hematocrit on the Cake Collapse Temperature of Lyophilized Red Blood Cells

Measurement of Cryoprotectant Permeability in Adherent Endothelial Cells and Applications to Cryopreservation

Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol

Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells

Membrane permeability properties of human granulocytes

Optimization of Cryoprotectant Loading into Murine and Human Oocytes

Investigating the Potential for Cryopreservation of Human Granulocytes with Concentrated Glycerol

Mathematically optimized cryoprotectant equilibration procedures for cryopreservation of human oocytes

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