The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
The economically viable production of value added fuels and chemicals from lignocellulosic feedstocks hinges on our ability to quickly and efficiently transform structural carbon molecules to end products. The sugars in lignocellulosic biomass are held primarily within cellulose and hemicellulose. Cellulose is composed primarily of glucose monomers which are quickly...
Atmospheric pressure changes do not stop at the permeable snow surface but rather propagate into it. These pressure changes range from high-amplitude, low-frequency events caused by seasonal cycles and synoptic weather systems to small-amplitude, high-frequency events caused by topographic features and turbulence. The effect of pressure changes on interstitial air...
When hops are added to beer, varying degrees of hoppy aroma persist in the finished beer as a result of a number of factors. Dry-hopping is a technique whereby hops are added to beer post-fermentation to leverage the maximum aroma potential of the hop essential oils while minimizing bitterness contribution...
Student engagement with faculty is positively associated with increases in the retention and graduation rates of students enrolled in institutions of higher education. Although a considerable amount of research has focused on understanding the engagement experiences of students enrolled at four-year colleges and universities, little emphasis has been placed on...
Plethodontid salamanders have served as an informative vertebrate system for studying the role of chemical signals in facilitating social and reproductive behaviors. Individuals produce complex mixtures of chemicals from multiple glandular regions. In total, these secretions convey a wide variety of information, and are important for numerous inter- and intraspecific...
Although only a minority of introduced species become established and have noticeable consequences in their new communities, some can displace native species, alter food webs, and cause local extinctions. Studying these invasive species can provide new insights into basic ecological questions as well as inform management strategies. Pacific lionfish (Pterois...
Biological invasions have been identified as one of the prominent drivers of global environmental change. In particular, invasive predators typically have substantial negative effects on populations of native prey, even driving species to extinction in extreme cases. However, beyond direct predatory effects, little is understood regarding the specific mechanisms by...
Red clover (Trifolium pratense L.) seed yield can be affected by plant growth regulators (PGR) and irrigation; however, the effects of these factors on physiological maturity (PM), harvest maturity (HM), and seed quality are unknown. The objectives of this study were to: 1) determine how irrigation and trinexapac-ethyl (TE, a...
There are many links between exposure to environmental pollution and risks to human health. While advances in the fields of toxicology, exposure science, and environmental chemistry have shown light on many of these links, many more research challenges remain. One major challenge is how to accurately characterize the toxicity of...
Multiple Sclerosis (MS) is an autoimmune condition that affects the central nervous system, and impacts the lives of over 400,000 individuals in the US. These individuals face unpredictable relapses of disabling conditions, are less active and experience poorer quality of life than the general population. Health professionals are challenged to...
The implementation and construction of Mechanically Stabilized Earth (MSE) walls has undergone substantial expansions in recent years, owing to its relatively low cost, ease of construction, and high efficiency compared to conventional retaining methods. As a result, MSE walls are being constructed to greater heights with complex features (e.g. multiple...
The multifaceted role of the environment in regulating the structure and dynamics of biological communities has long fascinated ecologists and motivated much debate and research. Now, in a time of accelerated global changes due to human impacts, the need to understand how the environment shapes communities has gained new urgency....
There is increasing awareness that human activities are altering the ways that natural systems operate and that local shifts in species composition and abundance can lead to abrupt and irreversible global change. Therefore, understanding the processes that buffer biological communities from critical shifts and how our actions affect natural stabilizing...
The goal of dissertation research was to use geochemical, statistical and geological methods to constrain and understand climate variability over several different time scales. Specifically, I have addressed three questions regarding past climate change: (1) how does the record of Irish cirque glaciers constrain the dimensions of the Irish Ice...
Despite more than two centuries of exploration, including more than six million deep wellbores with depths exceeding 40,000 feet in some parts of the world, our ability to constrain subsurface processes and properties remains limited. Characteristics of the subsurface vary and can be analyzed on a variety of spatial scales....
Today nano scale materials are being used for wide range of applications. One promising topic in nano scale materials is using them for reinforcement of different polymeric materials to reach desirable stiffness properties. The stiffness of these materials is mostly found experimentally because the nanocomposite community lacks a decent analytical...
Energy consumption has become a great deal for cloud service providers due to financial as well as environmental concerns. Studies show that cloud servers operate, most of the time, at only between 10% and 50% of their maximal utilizations. These same studies also show that servers that are kept ON...
Cognitive radio technology emerges as a promising solution for overcoming shortage and inefficient use of spectrum resources. In cognitive radio networks, secondary users, which are users equipped with cognitive radios, can opportunistically access spectrum assigned to primary users, the spectrum license holders. Although it improves spectrum utilization efficiency, this opportunistic...
The rapid scaling of network bandwidth and data center throughput has motivated the wide adoption of high speed transceivers. Silicon photonics (Si-Photonic) is one of the most promising techniques to realize tightly integrated optical transceivers for next-generation high speed I O standards. This dissertation focuses on the design techniques of...
Because of disease, industrialization, and regional conflicts, young widowhood is an expanding phenomenon in many parts of the world. Young widows face many challenges that can lead to mental health problems. The young widows have to make adjustments to fit their new roles as bread winners of the families, having...
Optimization of reservoir operation is involves various competing objectives for a scarce resource (water). To find the optimal operation of reservoirs, it is essential to consider multiple objectives simultaneously. There are various sources of uncertainty associated with the reservoir operation problem that should be considered as well.
The overarching goal...
Spintronics is an emerging field of research wherein the electron spin instead of, or along with, the conventional electron charge may be utilized in developing next generation logic devices. One of the most promising aspects of spintronics is that information transfer may be achieved by spin waves in logic devices,...
Zinc Sulfide (ZnS) thin film, with a wide band gap, has been used for many applications, such as buffer layer for CIGS solar cells, light emitting diodes and thin film electroluminescent devices.
In this work, ZnS thin films were prepared using two different deposition processes. In the first method, ZnS...
Phytoplankton are a sentinel class of organisms in the marine environment. Through their photosynthetic activity in sunlit waters worldwide, phytoplankton shape the health and productivity of marine ecosystems and impact the global climate. In this work a range of ocean sensing technologies (via ships, surf zone sampling, moorings, gliders, and...
Spalting fungi show strong potential as natural colorants on a variety of materials, specifically textiles and wood. Four species of spalting fungi are currently utilized in this manner: Scytalidium cuboideum, which produces a red pigment called draconin red, Scytalidium ganodermophthorum, that produces an unknown yellow pigment, and Chlorociboria aeruginosa and...
Meadowfoam (Limnanthes alba) is an oilseed crop with unique chemical characteristics. The ability to identify physiological maturity (PM) and harvest maturity HM of the crop would permit timely harvest of high quality seeds. However, changes of oil contents, fatty acid profile and glucosinolate contents, as well as seed quality during...
Oceanic uptake of rising anthropogenic CO₂ emissions has caused the emergence of ocean acidification as a major threat to marine ecosystems worldwide. Along eastern boundary current systems, seawater is naturally acidified due to coastal upwelling of low pH seawater from depth. Compounded by ocean acidification, upwelling regions are expected to...
The early marine phase following freshwater emigration has been identified as a critical period in salmonid (Oncorhynchus spp.) life history, characterized by high but variable mortality. Consistent with the “growth-mortality” and “bigger-is-better” hypotheses, at least some of the mortality during the critical period appears to be size-dependent – with smaller...
In order to more effectively design large, complex systems, risk must be accounted for throughout the design process. A systematic way to account for risk is by using system model simulation. During the conceptual design phase, functional models can be drawn to help the engineer design their system's functional architecture....
A fundamental difference between prokaryotic and eukaryotic cells is the presence of membrane bound organelles in eukaryotes. The dynamics of membrane trafficking within the cell are responsible for everything from intercellular communication and cell homeostasis, to mitosis, cell migration, and differentiation. These processes require exocytosis and compensatory endocytosis, often working...
There are more than 87,000 chemicals in current use with little to no toxicity information available. Assessing such a large number of chemicals using traditional methods would take an unreasonable amount of time and money, and require the use a large number of animals. The incorporation of high-throughput in vivo...
The Pacific Northwest (Oregon and Washington in the United States and British Columbia in Canada) is one of the major producers of red raspberry (Rubus idaeus L.) and blueberry (Vaccinium corymbosum L.) in the world. The expansion of growing area with these crops has resulted in the emergence of new...
Software testing is a very important task during software development and it can be used to improve the quality and reliability of the software system. One potential way to reduce the cost and increase the efficiency of software testing is to generate test data automatically. Search-based approaches successfully generate unit...
Bullying cultivates insecurity and fear while generating attention for the perpetrator and degrading the student-victim in front of others. The act erodes the self-efficacy and identity of minority students more specifically and produces a hostile climate that undermines the learning and social development to all affected. Despite the pervasive negative...
The purpose of this dissertation was to add to the professional literature in counselor education and supervision through inquiry and synthesis of research among online teaching, online counseling, and online communities of practice through the supervision lens of the Discrimination Model. A unique, research-informed framework for synchronous online supervision (i.e.,...
This study documented the influence of the completion agenda on decision-making by community college career and technical education (CTE) deans. Ten career and technical programs deans were identified as the sample for the study. Washington State was selected because of the Student Achievement Initiative that was developed as a completion...
It is uncertain how predicted changes in climate will impact vegetation responses and plant species’ distributions because the physiological mechanisms underlying thresholds for damage are not well understood, and responses to stress vary by functional type and developmental stage. Thus, it is crucial to investigate physiological responses to heat and...
Coastal marsh vegetation is an important component in maintaining marsh stability that is threatened by changes in sedimentation, sea level rise, natural and anthropogenic disturbances, and competition from invasive species. Vegetation has been demonstrated to reduce wave energy, increase sedimentation, and decrease erosion in tidal environments under a range of...
Research on the population genetics of microbial organisms requires the use of specialized analyses designed for clonal organisms to avoid violating the assumptions of traditional population genetic models. The tools necessary for performing these analyses existed as a set of unrelated software with non-overlapping capabilities and did not cover all...
Hearing loss is one of the most common defect, affecting 360 million people worldwide due to several factors including congenital, present at or soon after birth or acquired with age. Congenital hearing loss affects 32 million children in the world. The economic impact of hearing loss is estimated to cost...
Clostridium perfringens type A strains are one of the main causative agents of gastrointestinal (GI) diseases in human and can cause both food poisoning (FP) and non-food-borne (NFB) diseases. Several factors contribute to the pathogenesis of C. perfringens type A strains including the wide presence of C. perfringens spores with...
Marine mammals are top predators that are essential for the health and function of our oceans. These top predators are often affected by various factors that can be detrimental to their populations. Therefore, there is a need to evaluate undetermined causes of deaths and to better understand known diseases in...
The Endangered Species Act of 1973 (ESA) is considered by many to be among the most powerful and most contentious environmental laws in the United States. Persistent challenges to the Act’s implementation make reaching conservation goals problematic. Most notably, the very nature of the law—providing protections for species already at...
Radio frequency identification (RFID) has long been regarded as an automated data collection technology that promises solutions to inefficiencies found in supply chains such as “just-in-case” inventory stock, inventory obsolescence, stockouts, and theft. However, the disappointingly slow return on RFID investment has forced companies to examine and redefine RFID initiatives....
Machine learning models for natural language processing have traditionally relied on large numbers of discrete features, built up from atomic categories such as word forms and part-of-speech labels, which are considered completely distinct from each other. Recently however, the advent of dense feature representations coupled with deep learning techniques has...
Drought and mandatory water restrictions are limiting the availability of irrigation water in many important blueberry growing regions and new strategies are needed to maintain yield and fruit quality with less water. Three potential options for reducing water use, including deficit irrigation, irrigation cut-offs, and crop thinning, were evaluated for...
Porous carbon is indispensable in modern technology applications. It is used for energy storage, gas separation, water purification, catalyst support, and chromatography. The diversity of its applications stems from its unique properties, including high specific surface area, tunable pore volume, and chemical stability. Specifically, the large surface area provides high...
Piezoelectric materials convert mechanical strain into a dielectric displacement, as well as the converse, allowing these materials to be used as sensors, actuators, and transducers. Currently, lead zirconate titanate (PZT) is the primary material used in these applications. Due to environmental toxicity and safety concerns associated with Pb, development of...
The overall concept of reducing laboratory operations to a scale that fits on a single microfluidic chip has been an attractive area of research over the last several decades. Despite a surge in research, few commercial success stories have been written. Lab-on-a-chip technologies have the capability to be cost effective...