The spectrophotometric titration of the tyrosine residues,
their reaction with N-acetylimidazole, and the effect of pH on the
thermally induced conformational transition of ribonuclease T₁ have
been investigated. The results of spectrophotometric titration in
0.20 M KCl/0.001 M sodium phosphate suggest that titration of the
tyrosine residues occurs in two...
The halophilic alkaline phosphatase of Halobacterium
salinarium has been purified to a specific activity of 3,000-3,200
units per mg of protein. The purification scheme consisted of
acetone and ammonium sulfate fractionations, and molecular sieving
and adsorption chromatography on Sephadex G -50. The enzyme was
stabilized during purification by the presence...
Euphausia pacifica lives for a period of approximately one year,
disappearing from the catches at a size of about 22-24 mm. Spawning,
which occurs mainly inshore, extends from June through December
but generally is most prominent in the autumn months.
Average growth is calculated to be approximately 2.0 mm per...