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Filtering by: Creator Khanna, Satya Pal Degree Field Animal Science

Purification and characterization of recombinant calpain-5

Creator:

Wang, Mei-Chuan

Abstract:

Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....

Resource Type:

Dissertation

Full Text:

homology in Domain I (a long N-terminal region) and III (Pal homologous domain; PBH) (Sorimachi et al
Effect of Agronomically Selenium Biofortified Hay on Oxidative Status, Metabolic and Inflammatory Biomarkers, and Immune Response of Transition Primiparous Dairy Cows and Se Transfer and Glutathione Peroxidase Activity in their Calves

Creator:

Jaaf, Shana S.

Abstract:

During the peripartum period (3 weeks before through 3 weeks after calving, a.k.a. “transition”), high producing dairy cows experience, among others, , oxidative stress and immune suppression that compromise performance and increased incidence of diseases.Among trace minerals, supplementation of Selenium (Se) can help to improve the transition by enhancing glutathione...

Resource Type:

Dissertation