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Filtering by: Creator Sterner, Thomas Degree Field Biochemistry and Biophysics

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Creator:

Weber, Shane Crawford

Abstract:

I examined, in three separate studies, the active chromatin structure of the yeast, Saccharomyces cerevisiae. Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted by 0.25...

Resource Type:

Dissertation

Full Text:

; Noll, 1974; Kornberg & Thomas, 1974; Olins & Olins, 1974) began a revolution in the understanding of
Effects of nucleosomes on transcription by polymerase I in a reconstituted system

Creator:

Georgel, Philippe, 1961-

Abstract:

The aim of this study was to gain more information about the interactions between DNA and the histone octamer during the process of transcription. This work used a pUC8 plasmid derivative that contained the core promoter region of the RNA polymerase I of Acanthamoeba castellanii, placed upstream of four repeats...

Resource Type:

Dissertation

Full Text:

(for review see Thoma, 1992). The basic question is whether nucleosomes position randomly or exhibit a