In this dissertation, I present the work of developing more efficient and economical techniques for fragmenting large bio-molecules, and ultimately making mass spectrometry a more powerful tool in proteomics, DNA sequencing, and other areas of biomolecular analysis.
Aiming at lowering the installation and operating cost, and delivering electron capture dissociation...
The first part of this thesis describes a study of the
characteristics of oxidation of a 1:2 iron(III):thiolate system in
methanolic solution and in water. This system was investigated as a
potential analog for the cysteine- and cysteamine-dioxygenase catalyzed
0₂-oxidation of cysteine and cysteamine, respectively, to the
corresponding sulfinic acids....
The observed and calculated structural parameters of three systems
of phosphatidylcholine bilayers in fully-hydrated multilamellar
vesicles (MLVs) were determined using x-ray diffraction techniques.
The analysis used the x-ray reflections from the low-, intermediateand
wide-angle regions of the diffraction patterns in the subgel, gel,
rippled and fluid phases to determine the...