This study aimed to explore the relationship between urinary cortisol levels and proximity-seeking behavior in shelter cats and the impact of socialization programs on these two factors. A Spearman’s correlation test found no correlation between cortisol levels and proximity-seeking behavior in shelter cats. Fisher’s 2x2 tests confirmed that the changes...
The loss of all deuterium labels from previous feedings of [2-²H]-,
[3,3-²H₂]-, and [2,3,3-²H₃]arginines had indicated the possible intermediacy
of β-hydroxy- and β-ketoarginine (30 and 31, respectively) in the
biosynthesis of the streptolidine portion of streptothricin F (la).
In order to confirm or refute this evidence, D,L-[2,3,3,5,5-²H₅]-
arginine (32) was...
E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...
Fluorinated alkyl substances, which can be persistent, toxic, and
bioaccumulative, have been quantitated in many densely populated and
remote regions, including in air, surface waters, groundwater, and biota;
however, little is known about their transport or behavior in the environment.
Wastewater effluent is one of the principal routes for introducing...