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  • Coliform bacteria associated with redwood and drinking water emanating from redwood tanks

    Creator:
    Morrow, Jan Elaine
    Abstract:
    Coliform contamination of a rural water system led to a research project investigating the problems surrounding storage of drinking water in redwood tanks. Field studies of redwood tanks presently in use showed development of large coliform populations (notably, Klebsiella and Enterobacter species) soon after tanks were put into use. This...
    Resource Type:
    Masters Thesis
    Full Text:
    Edwards and Ewing (8). A greater percentage of Klebsiella isolates were shown to produce indole and H 2S

  • Impacts of microbial chemical interactions on marine plankton physiology

    Creator:
    Moore, Eric R.
    Abstract:
    The bioavailability of chemical compounds in the marine environment fundamentally influences the growth and physiology of microorganisms. Organic and inorganic chemicals that are produced by some marine plankton can be consumed by other plankton for energy production, growth, or to initiate essential physiological processes. Cultures of the diatom Thalassiosira pseudonana,...
    Resource Type:
    Dissertation
    Full Text:
    Abstract approved: ______________________________________________________ Kimberly H. Halsey The

  • Studies to improve lactic acid bacterial starter cultures

    Creator:
    Khosravi, Lewa
    Abstract:
    Three commercial sausage starter cultures were grown in an internal-pH-controlled medium (PHASE 4) and in a soluble phosphate-buffered medium. The cultures were tested by comparing viable counts before and after freezing at -40 C or freeze-drying and subsequent storage for 3 months in the frozen or dried state. Strains varied...
    Resource Type:
    Dissertation
    Full Text:
    in an internal-pH-controlled medium (PHASE 4) and in a soluble phosphate-buffered medium. The

  • The effect of temperature and hydrostatic pressure on protein, ribonucleic acid and deoxytibo-nucleic acid synthesis by Vibrio marinus, an obligate psychrophile

    Creator:
    Albright, Lawrence John
    Abstract:
    The marine psychrophilic bacterium Vibrio marinus MP-4 possessed a maximum temperature and hydrostatic pressure of 20 C and 425 atm for growth. The effects of temperatures of 21 and 25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on protein, RNA and DNA synthesis by V. marinus...
    Resource Type:
    Dissertation
    Full Text:
    marinus MP -4 possessed a maximum temperature and hydrostatic pressure of 20 C and 425 atm for growth

  • Preparation, evaluation, and use of lyophilized, concentrated dairy starter cultures in cheese and yogurt manufacture

    Creator:
    Speckman, Calvin Albert
    Abstract:
    Cultures of Streptococcus lactis and Streptococcus cremoris were grown in sterile 11% nonfat milk (NFM) at 32 C for 17 hr with the pH maintained at 6. 3 with sodium carbonate. The cells were harvested and resuspended in a medium of 5% gelatin, 5% sodium citrate, 2% monosodium glutamate and...
    Resource Type:
    Dissertation
    Full Text:
    culture; this activity resulted in a period of 5 1/4 hr from milk inoculation to milling in Cheddar

  • Factors affecting mitochondrial respiration in yeast

    Creator:
    Hough, Susan Carol
    Abstract:
    Two experimental approaches have been used to investigate the interaction of sterols with respiration in yeast. Cells were tested with an antifungal sterol, azasterol, and a mutant was obtained with an altered sterol composition. Polarographic measurements were done on mitochondria for both experiments. The effects of azasterol on mitochondrial respiration...
    Resource Type:
    Masters Thesis
    Full Text:
    the presence of substrate and ADP) by the polarographic slope of state 4 respiration (mitochondria in

  • Internal pH control starter media: use in culture preservation and for multiple strain growth

    Creator:
    Shigeno, Debra S.
    Abstract:
    Several bulk starter media, consisting of two experimental blends and four commercial preparations, were examined for each medium's ability to grow, preserve, and/or recover lactic streptococcal starter cultures. Nonfat milk was used as a control. Results showed that cultures which had the greatest survival rate in frozen storage had been...
    Resource Type:
    Masters Thesis
    Full Text:
    greatest survival in liquid storage @ 4° C were those grown in a medium capable of furnishing high pH

  • Antimicrobial resistance and plasmid properties of Leuconostoc and group N Streptococcus

    Creator:
    Orberg, Paulo K.
    Abstract:
    A method for rapid isolation of plasmid DNA from group N streptococci was developed. Chief advantages of the method were simplicity, the utilization of microliter quantities of reagents, and the obtainment of preparations highly enriched for covalently closed circular plasmid DNA. The method was also applied to over 15 Leuconostoc...
    Resource Type:
    Dissertation
    Full Text:
    . Microbiol. 43: 1213-1215. 18. Kornberg, A. 1980. DNA replication, p. 464-466. W. H. Freeman & Co., San

  • Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

    Creator:
    Neal, William Daniel
    Abstract:
    A cell free extract from Saccharomyces cerevisiae containing the enzyme that causes reduction of the 24(28)methylene group on the side chain of ergosterol has been studied. Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme activity was increased in cells grown with ethanol as the substrate. The Km was...
    Resource Type:
    Masters Thesis
    Full Text:
    love and devotion TABLE OF CONTENTS I. INTRODUCTION Page 1 II. LITERATURE REVIEW 4 Early

  • Survival and persistence of Bacteroidales human and ruminant specific fecal markers and occurrence with fecal pathogens

    Creator:
    Walters, Sarah P.
    Abstract:
    Aquatic fecal contamination from non-point sources impairs environmental health and serves as a vehicle for transmission of waterborne disease, resulting in economic losses worldwide. Accurate methods of diagnosing fecal pollution and its source are needed to prevent human exposure, remediate pollution, and reduce economic impacts. In order to obtain this...
    Resource Type:
    Dissertation
    Full Text:
    ............................................................................................51 CHAPTER 4. PERSISTENCE AND GROWTH OF FECAL BACTEROIDALES ASSESSED BY BROMODEOXYURIDINE