The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV)
found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a
molecular weight of approximately 150 kDa and was dependent on divalent cations,
either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
Bdellovibrio bacteriovorus 100 and B. stolpii UKi-2 each produce
two extracellular proteases which attack Azocoll (collagen)
while B. starrii A3.12 produces at least four such proteolytic
enzymes. Under standard growth and assay conditions each Bdellovibrio
species produces a unique amount of proteolytic activity. The
decreasing order of enzyme units was...
Core samples from five sites in the Yaquina and Coos Estuary
were assayed for vertical distribution of total sulfides, percent
volatile solids, redox potential, particle size, total aerobic and
sulfate reducing bacteria. Higher total sulfide levels were found at
sites having more than four percent volatile solids, redox potential
below...
The transcriptional promoter sequences for the Rhizobium meliloti
nitrogen fixation genes nifA and nifB were cloned to a β-galactosidase gene
fusion plasmid vector and transferred by homologous recombination to a
specialized transducing phage. The promoter fusions were then transduced
to a recombination deficient strain of Escherichia coli as single-copy
lysogens...
The majority of our modern understanding of bacterial pathogenesis is based on the strategy that involves screening bacterial genomes for the presence of the genes encoding pathogenic factors, and analysis of these genes via forward and reverse genetics. Chlamydiae represent a unique group of pathogenic bacteria in which it is...
The genome of the nuclear polyhedrosis virus which infects the
larvae of the tussock moth, the lepidopteran, Hemerocampa
pseudotsugata, was isolated, identified as deoxyribonucleic acid, and
characterized in terms of its physical and biological properties. An
isolation procedure for the viral DNA was established which consisted
of the following steps:...
Six serotypes of calicivirus (San Miguel Sea Lion Virus types 4,6,7
and 13, Mink Calicivirus and Reptile Calicivirus) were plaque purified,
and two-dimensional gel electrophoresis fingerprints, of the ³²P-labeled
Ribonuclease T1 digested viral RNA, were performed for each serotype.
Fingerprints revealed several oligonucleotides with similar mobilities,
and therefore, possibly similar...
Pantoea vagans C9-1 (C9-1) is a biological control agent sprayed onto apple and pear trees during bloom for suppression of fire blight, a devastating bacterial disease caused by Erwinia amylovora. Prior bioinformatics studies of C9-1 focused on predicted phenotypes associated with the three native megaplasmids, pPag1, pPag2, and pPag3, and...
The dairy industry relies primarily on consistent acid production
by the lactic streptococci for the manufacture of certain cheeses
and fermented dairy products. Variation in these cultures due to
genetic exchange has not been thoroughly investigated. This study
was undertaken to determine if genetic homology exists within the lactic
group,...