Primary monolayer cultures of chick renal epithelial cells
grown on plastic coverslips were reversibly dissociated with the
chelating agent ethylenediaminetetraacetic acid (EDTA). The cellular
responses during dissociation and reaggregation in Hank's balanced
salt solution (BSS) were observed with phase optics and recorded
by time-lapse cinemicrography.
The epithelial cells grow as...
The presence of the etiologic agent of "salmon poisoning"
disease, Neorickettsia helminthoeca, was demonstrated in eggs of
the trematode vector, Nanophyetus salmincola. Three dogs were
given 100,000 and one dog 82,000 ground fluke eggs by intraperitoneal
injection. The four animals developed "salmon poisoning"
disease and died. One of these dogs...
The 13 species of shrimps studied for this thesis were collected
off the Oregon coast. The family Oplophoridae is well represented
in this area. Five of the seven known genera were identified.
Hymenodora frontalis, H. glacialis, and H. gracilis were described
and further differentiating characters were illustrated. Other members
of...
Developing sea urchin eggs (Strongylocentrotus purpuratus),
reared at 15° C, were subjected to single 15-minute treatments with
disodium versenate in calcium-free sea water, applied at 10-minute
intervals, from 4 minutes after insemination to the second cleavage.
These versene pulses produced specific degrees of retardation in
the mitotic process and delayed...