Levels of activity of glucose-6-phosphate- , 6-phosphogluconate- , and isocitric- dehydrogenase were measured in the
soluble and particulate fractions of heart, brain and liver at
several ages throughout the development of the chick embryo.
Compared to isocitric dehydrogenase, levels of glucosesphosphate-
, and 6-phosphogluconate- dehydrogenase are generally
low. This suggests...
The cytoplasmic isozyme of malate dehydrogenase (s-MDH) has been isolated from brine shrimp nauplii to a state of high purity with a protocol involving ammonium sulfate fractionation and chromatography on DEAE-cellulose, followed by sequential passes over cellulose phosphate columns at pH 5.8 then 5.0. The purified enzyme is free of...
Butane-grown Pseudomonas butanovora oxidized butane by a soluble butane monooxygenase through the terminal pathway yielding 1 -butanol as the predominant product. Alcohol dehydrogenases (ADHs) involved in butane oxidation in P. butanovora were purified and characterized at the biochemical, genetic and physiological levels. Butane-grown P. butanovora expressed a type I soluble...
