Tn5 mutagenesis of apdA (for antibiotic production) and deletion of
gacA (for global antibiotic and cyanide) resulted in the same pleiotropic phenotype in
Pseudomonas fluorescens (i.e. production of an array of secondary metabolites
including the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol as
well as a tryptophan-side-chain oxidase, hydrogen cyanide, and an...
A major factor in the ability of Pseudomonas fluorescens Pf-5 to act as a biological control agent is its production of antibiotics, including pyoluteorin (PLT), 2,4-diacetylphloroglucinol (2,4-DAPG) and pyrrolnitrin (PRN). The data provided in this thesis demonstrate that the presence of any of these antibiotics in the extracellular milieu affects...
Pseudomonas is a diverse genus of Gram-negative bacteria that includes pathogens of plants, insects, and humans as well as environmental strains with no known pathogenicity. Pseudomonas fluorescens itself encompasses a heterologous group of bacteria that are prevalent in soil and on foliar and root surfaces of plants. Some strains of...