The central purpose of this study was to identify the
competencies of a predeparture orientation program as determined by
business/industry personnel and personnel from institutions
representing the U.S. Agency for International Development. An
additional objective was to examine the organizational framework of
existing predeparture programs to identify instructional
methodologies, administrative...
In order to produce infectious virus progeny, vaccinia virus (VV)
undergoes morphogenic proteolysis to regulate the structural rearrangements of
virus particles. Several of the major structural precursor proteins of VV are
cleaved at a conserved Ala-Gly-X (where X is any amino acid) motif by the VV
I7L core protein proteinase...
Replication of vaccinia virus in the cytoplasm of the
infected cell occurs under the direction of virally encoded gene
products. Expression of approximately 200 viral genes follows a
highly regulated temporal scheme which can be followed
biochemically and morphologically during the assembly process.
In order to dissect this complex genetic...
Local adaptation in plants may hold the key to understanding the level of resilience of an ecosystem and probability of persistence of a species in the face of rapid anthropogenic changes in climate and disturbance regime. Clonal species are especially important in wetlands, one of our most productive and vulnerable...
Several laboratories have shown that the VV
replication cycle is dependent on the host cell nucleus.
The basic biological observation is that if the host cell
nucleus is physically removed by cytochalasin B-mediated
enucleation, functionally inactivated by UV-irradiation, or
selectively blocked with a-amanitin, the results are the
same: W is...
Vaccinia virus gene expression is characterized by tight temporal
regulation. Viral early transcription begins within the cytoplasm upon
infection; three hours later, early genes are repressed, DNA replication
occurs and late gene expression commences. As an approach toward
understanding the mechanisms governing vaccinia late gene expression,
I have undertaken an...
Thymidine kinase is a key enzyme in the nucleotide salvage pathway,
catalyzing the production of dTMP from thymidine and ATP. In order to
identify the structural features of the TK protein and/or primary amino
acid sequences which contribute to the catalytic and regulatory activities of
this enzyme, an in vitro...
Vaccinia virus (VV) is a large double-stranded DNA virus that is a prototypic member of the orthopoxvirus family. Previous works has showed that three of the major structural proteins found within the mature VV virion core 4a, 4b, and 25K are produced from higher molecular weight precursors at late times...
We identify all translation covers among triangular billiards surfaces. Our main tools are the J-invariant of Kenyon and Smillie and a property of triangular billiards surfaces, which we call fingerprint type, that is invariant under balanced translation covers.