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Filtering by: Creator Rinaldi, Nicole A. Has journal sim ACS Synthetic Biology

Antigen Binding and Site-Directed Labeling of Biosilica-Immobilized Fusion Proteins Expressed in Diatoms

Creator:

Ford, Nicole R., Hecht, Karen A., Hu, DeHong, Orr, Galya, Xiong, Yijia, Squier, Thomas C., Rorrer, Gregory L., and Roesijadi, Guritno

Abstract:

The diatom Thalassiosira pseudonana was genetically modified to express biosilica-targeted fusion proteins comprising either enhanced green fluorescent protein (EGFP) or single chain antibodies engineered with a tetracysteine tagging sequence. Of interest were the site specific binding of (1) the fluorescent biarsenical probe AsCy3 and AsCy3e to the tetracysteine tagged fusion...

Resource Type:

Article

Full Text:

Biosilica-Immobilized Fusion Proteins Expressed in Diatoms Nicole R. Ford,† Karen A. Hecht,† DeHong Hu