Index Catalog // ScholarsArchive@OSU

Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

Creator:: Codding, Sara Jone
Abstract:: The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
Resource Type:: Dissertation
Full Text:: :________________________________________________________ Colin P. Johnson The focus of this dissertation was to understand protein function

Initial in vitro and in vivo Characterization of the Membrane Trafficking Protein Fer1L6

Creator:: Wolk, Chelsea
Abstract:: A fundamental difference between prokaryotic and eukaryotic cells is the presence of membrane bound organelles in eukaryotes. The dynamics of membrane trafficking within the cell are responsible for everything from intercellular communication and cell homeostasis, to mitosis, cell migration, and differentiation. These processes require exocytosis and compensatory endocytosis, often working...
Resource Type:: Dissertation
Full Text:: Colin P. Johnson Abstract A fundamental difference between prokaryotic and eukaryotic cells is

Characterizing the Functional Properties of Otoferlin, Essential for Neurotransmission in Inner Hair Cells of the Cochlea

Creator:: Narayanappa, Murugesh
Abstract:: Hearing loss is one of the most common defect, affecting 360 million people worldwide due to several factors including congenital, present at or soon after birth or acquired with age. Congenital hearing loss affects 32 million children in the world. The economic impact of hearing loss is estimated to cost...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Colin P. Johnson Hearing loss is one of the most common defect

Cloning the zebrafish otoferlin b wildtype and a transmembrane mutant to characterize changes in cellular localization

Creator:: France, Rebecca A.
Abstract:: Otoferlin is a protein that is essential in the process of hearing. Mutations in otoferlin are associated with profound recessive deafness. The long-term goal of the Johnson lab is to understand how otoferlin contributes to hearing and why certain mutations result in deafness. This project sought to characterize the difference...
Resource Type:: Honors College Thesis
Full Text:: :_____________________________________________________ Colin P. Johnson Otoferlin is a protein that is essential in the process of hearing

Characterization of Fer1L6 in the mouse C2C12 cell line

Creator:: Huegel, Jacob T.
Abstract:: Fer1L6 is an uncharacterized member of the ferlin family of proteins, a group of large, calcium sensitive proteins with emerging roles in vesicle trafficking and various disease states. The presence of Fer1L6 has been identified within the C2C12 cell line of mouse skeletal muscle. To determine the protein’s subcellular location...
Resource Type:: Honors College Thesis
Full Text:: C2C12 Cell Line. Abstract Approved: _________________________________________ Colin P. Johnson

Characterization of Ferlin-1-Like 6 (Fer1L6) Through Lipid Membrane Interactions

Creator:: Matlapudi, Susmitha
Abstract:: The ferlin protein family regulates membrane trafficking events for a wide range of cellular and physiological processes in eukaryotic organisms. Structurally, ferlins are distinguished by containing five to seven C2 domains with a single-pass transmembrane domain at the C-terminus. In mammals, there are 6 ferlin genes, including Fer1L6, which confer...
Resource Type:: Honors College Thesis
Full Text:: approved:_____________________________________________________ Colin P. Johnson The ferlin protein

Conformational Consequences: Calcium Regulates the Association Between Dysferlin C2A & PI(4,5)P2

Creator:: Otto, Shauna C.
Abstract:: Dysferlin is a ∼230 kDa terminally anchored membrane protein that is ubiquitously expressed, but is particularly enriched in skeletal and cardiac muscle tissue. Mutations covering the length of the protein have been linked to muscle wasting diseases including limb-girdle muscular dystrophy and Myoshi myopathy. Dysferlin has been shown to play...
Resource Type:: Dissertation
Full Text:: approved: Colin P. Johnson Dysferlin is a ∼230 kDa terminally anchored membrane protein that is ubiqui

Characterization of Fer1L6 lipid binding properties and its expression in cell cycle

Creator:: Chau, Trisha J.
Abstract:: Ferlins are a family of calcium-sensitive proteins involved in fusion and fission membrane trafficking events. There are six ferlin members: dysferlin, otoferlin, myoferlin, Fer1L4, Fer1L5, and Fer1L6. Previous research shows the first three ferlins play a pathophysiological role in humans, while the other three remain uncharacterized. Fer1L6 is one of...
Resource Type:: Honors College Thesis
Date Created:
Full Text:: :_____________________________________________________ Colin Johnson Ferlins are a family of calcium-sensitive proteins involved in fusion and

Establishing Larval Zebrafish as an In Vivo Model Organism for Characterizing the Roles of Otoferlin, a Sensory Hair Cell Protein Essential for Hearing

Creator:: Chatterjee, Paroma
Abstract:: Auditory defects and disorders are prevalent at all ages and affect 8% of the population in developed nations including newborns and children. Congenital hearing loss is the most common birth defect and it is estimated that 1 in 1000 children are affected by deafness at birth or before the onset...
Resource Type:: Dissertation
Full Text:: : _________________________________________ Colin P. Johnson Abstract Auditory defects and

Single-molecule in vitro Characterization of Otoferlin

Creator:: Hams, Nicole J.
Abstract:: Congenital hearing loss is a common disorder and over 60 mutations in the sensory hair cell protein, otoferlin, have been linked to pre-lingual deafness. Although otoferlin is essential for hearing, the large size and low solubility of the protein have limited approaches to study its function on a molecular-level. To...
Resource Type:: Dissertation
Full Text:: of Otoferlin Abstract Approved: Colin P. Johnson Congenital

ScholarsArchive@OSU

Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

Initial in vitro and in vivo Characterization of the Membrane Trafficking Protein Fer1L6

Characterizing the Functional Properties of Otoferlin, Essential for Neurotransmission in Inner Hair Cells of the Cochlea

Cloning the zebrafish otoferlin b wildtype and a transmembrane mutant to characterize changes in cellular localization

Characterization of Fer1L6 in the mouse C2C12 cell line

Characterization of Ferlin-1-Like 6 (Fer1L6) Through Lipid Membrane Interactions

Conformational Consequences: Calcium Regulates the Association Between Dysferlin C2A & PI(4,5)P2

Characterization of Fer1L6 lipid binding properties and its expression in cell cycle

Establishing Larval Zebrafish as an In Vivo Model Organism for Characterizing the Roles of Otoferlin, a Sensory Hair Cell Protein Essential for Hearing

Single-molecule in vitro Characterization of Otoferlin

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