Optimization of preparative nonlinear chromatography was carried out for
the first time for a biomolecule mixture. Conventional wisdom on optimization,
which roots from analytical chromatography, dictates optimizing resolution in an
analytical column and obtaining similar separation in a large column for isolation.
Such a method of optimization significantly under uses...
This study compared the growth of Laminaria saccharina female gametophyte filamentous cell suspension cultures in a stirred-tank photobioreactor under batch and fed-batch nutrient addition modes over a 48-day cultivation period. Cultures were grown on GP2 artificial seawater medium (0.75 mM nitrate, N:P = 16:1) at pH 8.3 without iron or copper. Total...
Gradient elution chromatography is frequently used for the preparative separation
of peptides and proteins. Separations at high loadings are often avoided because peaks
become asymmetrical and saturate the detector. However, non-linear interactions which
become important at high loadings may actually improve the separation with greater
concentrations of the product being...