Part 1:
White muscle disease (WMD), a degenerative muscle disease brought on by a deficiency of the essential trace nutrient selenium, is thought to be mediated by the protein Selenoprotein W (SeW). In its relationship with WMD, SeW, like many selenoproteins, binds to glutathione, serving some purpose in redox mechanisms...
Heavy metals have historically been popular for use in cosmetics manufacturing due to the colorful pigmentation of heavy metal compounds. In the case of sindoor, a cosmetic used by married Hindu women for religious purposes, mercury sulfide, lead tetroxide, and lead chromate are compounds suspected to still be involved in...
Anaerobic digestion is a common terminal step in wastewater treatment systems and produces valuable by-products such as biogas, a combination of carbon dioxide and methane. Biogas can be used to off-set energy costs, and along with the anaerobic co-digestion of fats, oils, and greases (FOG) has been found to increase...
Obesity is achieved at the point where fat accumulation begins to exert deleterious effects on an organism. The human body accrues fat when there is an energy imbalance occurring, where caloric intake exceeds expenditure.Many anti-obesity drugs used in the past have had undesirable side effects, and currently there is a...
STEM education is delivered to K12 audiences by Pre-college Programs across the nation, many of which serve underrepresented and underserved students as part of their core missions. While there is robust attention to STEM curriculum development and college connection events in such programs, few have social-cultural aspects of learning as...
As scientific technologies and techniques have improved in past decades, it has become
possible to quickly collect unprecedented quantities of experimental data. In the study of gene expression, for instance, datasets comprising tens of thousands of variables and hundreds of treatments can be produced in a matter of months. When...
Modifications of histone tail amino acid residues are necessary for many reactions involving chromatin. These processes can produce transcriptionally active, euchromatic, or inactive, heterochromatic, segments of DNA. The formation and maintenance of facultative heterochromatin is critical for the proper growth and development of many eukaryotes. Euchromatin can be changed into...
Hatcheries are used to produce salmon to augment the number of fish available for harvest, and to supplement wild populations that may be threatened with extinction. Although traditional hatchery rearing methods can produce many fish, they can pose risk to wild populations because they tend to produce salmon with lower...
Abstract
Understanding the effects of climate change on forest ecosystems requires exploration of the relationships of different components of the system, such as the response of biota to local hydrology, temperature, precipitation, and elevation. The Long-term Ecological Research Program at the H. J. Andrews Experimental Forest, located in the Western...
Humans and rodents experience declines in reference (long-term) and working (short-term)
memory and cognitive flexibility during the aging process. Aging changes in GluN1 and
GluN2B subunits of N-methyl-D-aspartate (NMDA) receptors show a relationship to both
reference and working memory deficits. Sulindac, an anti-inflammatory drug, enhances working memory and NMDA receptor...
A pro-inflammatory agent, lipopolysaccharide, can mimic the effects of aging on spatial reference memory
E. R. ESCOBEDO and K. R. Magnusson, Dept. of Biomedical Sciences, Coll. of Vet. Med., Linus Pauling Institute, Oregon State University, Corvallis, OR 97331
Humans and rodents experience declines in reference (long-term), working (short-term) memory, and...
As in other species, it is possible that a homozygous red leafed (= redleaf) hazelnut (Corylus avellana L.) tree would have brighter red coloration than a heterozygote and thus be more desirable as an ornamental. However, 'Fusco Rubra' and `Rode Zeller' both carry incompatibility allele S6 (thus cross-incompatible) and are...
Separation and isolation of organic analytes from environmental matrices such as soils,
water, plant, or animal tissue has traditionally involved the use of liquid solvent extractions.
Solvent-based extraction methods are often time-consuming, difficult to automate, and use
expensive solvents that generate hazardous waste and working conditions. The need for a...
The nursery industry plays a critical role in Oregon's economy. An increase in wood-boring insect, or borer, activity has impacted the industry. The goal of this research is to determine key borer species in the nursery industry, flight times so targeted spray schedules can be established, and assess grower knowledge...
Amyotrophic lateral sclerosis (ALS) is characterized by the degeneration of motor neurons in the motor cortex and spinal cord. Recent evidence suggests that the purine receptor P2X7 (P2X7R) plays a central role in the pathology of ALS. Previous research indicates that P2X7R activation by oxidatively modified heat shock protein 90...
Age-related decline in the capacity of endogenous antioxidant systems
complicates pharmaceutical use by the elderly by potentially increasing the toxicity of
some compounds. Of particular interest is chemical detoxification via glutathione (GSH)
and attendant GSH-requiring detoxification enzymes, e.g. GSH peroxidases (GPX) and
GSH S-transferase (GST). These enzymes have been shown...
Lung cancer in humans is an adult-onset disease. It is the number one cause of cancer mortality for both men and women and has a poor (15%) survival rate. Biological markers of the disease would allow for early diagnosis and treatment. The specific aims of this research project were to...
Wheat is an important part of a healthy and balanced diet, requiring six servings per day to maintain a recommended 2000 Calorie intake (McRae, 2017). Because of this more land than almost any other kind of cereal is set aside for the purposes of growing wheat. To keep up with...
As part of the Botswana Global Learning Internship Program, a qualitative study was conducted regarding the current staffing conditions at the local village health clinic in Maunatlala, Botswana. This was a month-long project conducted over the summer of 2018. Students from Oregon State University lived in the village of Maunatlala...
Succinate dehydrogenase (Sdh) inhibitor fungicides, such as boscalid, are effective for the management of gray mold caused by the fungal pathogen Botrytis cinerea. Unfortunately, resistance to boscalid was common among isolates of the pathogen from small fruits grown in Oregon. Boscalid-resistance is commonly associated with mutations in Sdh, especially in...
Contamination of water sources due to the use of agricultural chemicals can be minimized through understanding the transport relationships for organic chemicals in soil environments. In this study we evaluated seven non-hazardous dyes that have properties which were consistent with application as surrogates to organic agro-chemicals to assess mobility through...
Northwest cherry cultivars including Prunus avium 'Bing', Prunus avium 'Rainier', Prunus avium 'Royal Anne' and Prunus cerasus 'Montmorency' were separated into epidermal tissue, flesh and pit and cryogenically milled with liquid nitrogen. These samples as well as whole samples from each variety were extracted with acetone followed by a chloroform...
Infertility is an issue affecting millions of women across the globe, yet the causes are not fully understood, and treatment options are often ineffective. The evaluation of reproductive hormones has been a common tool used by fertility clinics as a way to predict the ovarian response to treatment and gain...
Antibiotic resistance is an increasing problem in many species of bacteria today, with pathogens an important focus. Fecal contamination of shellfish is already a concern due to potential pathogens. This report examines the possibility of resistance gene transfer between microbes, due to fecal bacteria, within the oyster. In this study,...
Spotted wing drosophila, Drosophila suzukii, is a recently introduced, invasive pest in Oregon. It oviposits in near-mature and mature fruit, and thus is an important concern for the small fruit industry. A crop consultant observed that mature raspberry fruit with D. suzukii larvae do not show symptoms of molds as...
Western diets can influence behavior and gut microbiome due to the excessive intake of high fat and sucrose. Altering the microbiome can also influence the brain and behavior. The hypothesis that was tested was that diet-induced changes in the microbiome cause changes in cognitive abilities. A previous study showed learning...
Motivation
Relatively little is known about the evolution of mitochondrial genomes between Caenorhabditis species despite decades of research. Both the worldwide search for new nematode species and the effort to increase sequencing speeds require automated tools to quickly characterize and categorize large amounts of data.
Results
We developed an automated...
Bacterial endophytes have the potential to confer benefits to Douglas-fir ( Pseudotsuga menziesii ), for instance drought tolerance. Bacterial endophytes originating from seeds are especially important. This is because seed endophytes are more likely to be the first endophytes to get established within the young host plant. The goals of...
Bacterial endophytes have the potential to confer benefits to Douglas-fir (Pseudotsuga menziesii
), for instance drought tolerance. Bacterial endophytes originating from seeds are
especially important. This is because seed endophytes are more likely to be the first endophytes
to get established within the young host plant. The goals of...
Knowledge of Drosophila suzukii's preference for an attractive trap design and bait can be used to improve detection and management of this new invasive fruit pest. Desired trap and bait attributes include: high D. suzukii capture and species specificity; early-season detection prior to significant crop damage; higher capture of females...
Collaboration is crucial when advancing in scientific research. One person cannot
study all aspects of a system. That is why it is important for the scientific community to
communicate with each other and stay updated on research being conducted in other labs
around the world. In my research, I was...
Liquid manure lagoons are known to be sources of odor and environmentally damaging gas emissions. Land application of the manure slurry after storage can lead to detrimental nutrient runoff and leaching. Floating lagoon covers (biocovers) are one option for reducing emissions, but to date they have only been used to...
Biogas can be used to generate energy from a wide variety of sources. Wastewater treatment sludge is an effective source of waste to be used for generation of biogas to make energy which can be used to heat homes and run businesses. This would help decrease the use of fossil...
Saturated soils undergo reduced conditions as microorganisms consume oxygen and require other terminal electron accepting processes for the mineralization of soil organic carbon. Currently, the soil redox potential has not been considered as important when sampling these soils for Fe, Mn, and P04. Since Fe- and Mn-oxides are electron sinks...
Biological control is the action of predators, parasitoids, or pathogens to maintain
a pest organism's population at lower numbers than would occur in the absence of the
agent. Entomopathogenic nematodes can be used as biological control agents for insect
pests. These nematodes infect insects and release a toxic bacterium that...
Abstract
Current methods employed in the textile industry are unsustainable because they use an abundance of water in the dying process, and discharge the waste into the environment, causing adverse health effect on aquatic life and local communities. The technology that is in place to remove dye from textile effluents...
The prevalence of obesity and diabetes in the United States and globally has been rising in the last few decades and it continues to climb. One of the causes for this increase is the over consumption of sugars and/or carbohydrates. Among medications available to treat obesity and diabetes are the...
Beneficial insects could provide valuable pest control services on Oregon Christmas tree farms, but a limited amount of data exist that quantifies the type and abundance of beneficial insects on farms. This study surveyed beneficial insects on two farms that differed in their management strategies as well as other abiotic...
Beneficial insects could provide valuable pest control services on Oregon Christmas tree farms, but a limited amount of data exist that quantifies the type and abundance of beneficial insects on farms. This study surveyed beneficial insects on two farms that differed in their management strategies as well as other abiotic...
The following paper is an objective view on the viability of growing the truffle Tuber melanosporum in the Willamette Valley. Included in this study are the history of the truffle, biological cycle, habitat description, method of cultivation and an enterprise budget for commercial production in the Willamette Valley. The truffle,...
Greenhouse gases effects are a leading contributor to climate change; reducing harmful effects of these gases is important for future generations. Worldwide, household consumption makes up seventy-two percent of greenhouse gas emissions, followed by government consumption at ten percent, and investments at eighteen percent (Hertwich, 2009). In order to understand...
This study examines climatological influences, particularly that of snowpack, on tree growth and stable carbon isotope discrimination (Δ¹³C) from ~1980 to 2013 at two sites located in the upper reaches of the McKenzie River watershed of the Oregon Cascade Mountains. We tested the use of Δ¹³C values from latewood, corroborated...
This study examines climatological influences, particularly that of snowpack, on tree growth and stable carbon isotope discrimination (Δ¹³C) from ~1980 to 2013 at two sites located in the upper reaches of the McKenzie River watershed of the Oregon Cascade Mountains. We tested the use of Δ¹³C values from latewood, corroborated...
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
The decline of the Chinook Salmon has been a mystery of recent years due to their decline. It has been speculated that wild origin salmon have more vigor and survivability than hatchery reared fish. This is thought to be due to rearing conditions, possibly affecting sensory organ development. The sensory...
The decline of the Chinook Salmon has been a mystery of recent years due to their decline. It has been speculated that wild origin salmon have more vigor and survivability than hatchery reared fish. This is thought to be due to rearing conditions, possibly affecting sensory organ development. The sensory...
Clostridium perfringens type A isolates produce the spore-specific enterotoxin, CPE, upon sporulation in the gastrointestinal tract of humans and animals. Spo0A is a response regulator representing the major transcription factor for sporulation initiation in C. perfringens. In Bacillus subtilis, a multicomponent phosphorelay involving five histidine kinases (KinA-KinE), the intermediate response...
Cytochrome P450 1B1 (Cyp1B1) is a xenobiotic metabolizing enzyme, responsible for polycyclic aromatic hydrocarbon (PAH) oxidative metabolism. CYP1B1 metabolically activates several species of environmentally relevant procarcinogenic PAHs to reactive carcinogens. PAHs are aryl hydrocarbon receptor (AhR) agonists, leading to CYP1B1 upregulation. Though CYP1B1 is highly conserved across species, wild type...
COUP-TF interacting protein 1 (CTIP1) is a novel C2H2 zinc finger protein that mediates transcriptional repression of ARP1, a member of the COUP-TF subfamily of orphan nuclear receptors [1]. Here we show that CTIP1 interacts with heterochromatin-associated protein 1 (HP1) in vitro and in cells. This
interaction suggests the existence...
Vitiligo is a disease characterized by depigmented patches of skin. The disease is known to involve the loss of melanocytes in the basal layer of the skin in affected areas, but the underlying mechanisms are unknown. Recent evidence suggests oxidative stress and disruptions to antioxidant pathways contribute to an autoimmune...
Many Gram-negative bacteria use a type III secretion system (TTSS) to establish a relationship with a host. Pathogens can use the secretion system as a way to deliver proteins known as type III effectors (TTEs) into a host cell and block host defense. The variation and redundancy of the type...
Ubiquitous fine particulate matter (PM2.5) exposures significantly impact global public health, yet little is known about the mechanisms causing these known adverse health effects. Oxidative stress due to PM2.5 associated chemical constituents, such as polycyclic aromatic hydrocarbons (PAHs), is a proposed possible mechanism for PM2.5 mediated health effects. Variations in...
South American waterweed, Elodea densa (Planch.) Casp., and Coontail Ceratophyllum demersum L., were studied for their potential to take up chromium and zinc and as a possible indicator of arsenic, cadmium, copper, and lead pollution. Plants were collected from the Willamette River in Oregon and exposed for 48 hours to...
Anthopleura elegantissima (Cnidaria: Anthozoa) is an emerging model organism for studying the endosymbiotic relationship between dinoflagellate (genus Symbiodinium) and the cnidarian host. To be developed as a model organism, more genetic resources, like integrated genome and linkage map, are needed. An important validation for developing genomic resources is chromosome number....
Chlamydiae are obligate intracellular bacterial pathogens with a unique biphasic life cycle that consists of an infectious but non-dividing form, called elementary bodies (EB), and a noninfectious but metabolically active form, called reticulate bodies (RB). Chlamydia has an unusual process of cell division of which still remains uncharacterized subject matter;...
Summary
Recent research has identified Retinoid-X-Receptorα (RXRα), a nuclear receptor involved in ligand mediated transcription, as having a protective function against the malignant transformation of melanocytes after treatment with chemical carcinogens. This study used mice selectively lacking keratinocytic RXRα in combination with an oncogenic mutation, either Cdk4[Superscript R24C/R24C] or N-Ras[Superscript...
Saturated hydraulid conductivity (Ks) is a central soil physical property in determining water movement and contaminant transport through soils; hence modeling this system requires an accurate determination of K. Decimeter-scale KS measurements employing cores or infiltrometers are used frequently, but field-scale KS values are required to encompass the full range...
Raspberry bushy dwarf virus (RBDV) infects red raspberry and black raspberry worldwide. Infection causes reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields. While this crumbly fruit can still be used in juice, jam, and preserves, it cannot be used for the more valuable fresh and individually...
Llama reproductive physiology is unique when compared with other livestock species. Llamas carry 95-98% of all pregnancies in the left uterine horn. It is believed that there is a physiological difference between the uterine horns that contributes to this phenomenon. This study examined the uterine environment for protease and protease...
Current protein immobilization techniques lack defined control over protein orientation. Protein orientation is important for making biosensors and biomaterials that are sensitive and efficient and can be crucial for designing some devices such as immunosensors or direct electron transferring biocells. A new method of protein immobilization is proposed that takes...
Colorectal cancer (CRC), the third leading cause of cancer death in the USA, depends on early detection for patient survival. Early detection is improved with the identification of high risk individuals. Risk of CRC development is a complex interaction between an individual’s genetics and environmental exposures. Polycyclic Aromatic Hydrocarbons (PAHs)...
With over three billion pounds of berries grown nationally each year, strawberries are an important economic contributor to agriculture in the US (Grubinger, 2012). However the strawberry industry in Oregon has been in a period of transition over the past few years. Strawberry acreage, planted and harvested, as well as...
Global atmospheric carbon levels are continuing to rise from pre-industrial revolution levels. One of the main regulators of global atmospheric carbon levels, and thus climate, is the world's oceans. Unicellular marine cyanobacteria account for a large percent of total marine carbon fixation. We measured chlorophyll-specific ¹⁴C uptake rates (P[superscript b])...
Long noncoding RNAs (lncRNAs) are RNA molecules that are at least 200 nucleotides long and do not encode proteins. lncRNAs have roles in gene regulation, chromatin epigenetics, and molecular scaffolding. In light of mounting evidence implicating species-specific noncoding RNAs and gene regulatory mechanisms in species adaptations, it is reasonable to...
The bovine blastocyst is composed of a one-celled layer of trophectoderm
surrounding a fluid filled sac called the blastocoel and a ball of cells called the inner cell
mass (ICM). On Day 8 (Day 0=fertilization) in bovine embryos, endodermal cells leave
the ICM, migrate over the extracellular matrix (ECM) on...
Acetaminophen (APAP) is a commonly used analgesic and antipyretic drug that can cause liver injury, liver necrosis, and liver failure. APAP-induced liver failure (AILF) is associated with depletion of glutathione and the increased formation of APAP protein adducts from the toxic NAPQI metabolite. There is a hypothesized detoxifying pathway where...
We are developing a flavin-containing (Fmo) knock-out mouse for Fmo2 that will be utilized to elucidate the role of active human FMO isoform 2 (FMO2.1) in xenobiotic metabolism and toxicity. Since FMO2 is primarily expressed in lung this will provide information on how 27% of African-Americans and 5% of Hispanics...
Soil contains approximately 75% of the carbon pool on land - three times more than the amount stored in living plants and animals (Schlesinger 1999). Therefore, soils play a major role in maintaining a balanced global carbon cycle. Models of the soil carbon ecosystem assume a strong relationship between organic...
Chlorinated and non-chlorinated solvents are used in a variety of industrial processes and as a consequence, they have become environmental contaminants as well. Because of this, they lead to both occupational and environmental exposures. Exposure to these compounds can potentially result in various forms of neurological damage with a wide...
The western spotted cucumber beetle (Diabrotica undecimpunctata
undecimpunctata) and the western striped cucumber beetle (Acalymma trivittatum) are
pests of many crops, including vegetable, fruit, and nursery crops. Pesticides are
commonly used to control this pest. Trap and kill technologies are under development as
use in an alternative control for cucumber...
Squash varieties of Golden Delicious and Squash Hybrid NK 530 were grown in disk-till, strip-till, and no-till conservation tillage systems following a cover crop of'Monida' oats (Avena sativa L.) and common vetch (Vicia sativa L.). Tillage systems were evaluated for effects on squash emergence and yield, weed density and biomass,...
Sweet onions are valued for their unique flavor and praised for their health and dietary character. Onion sweetness is perceived as the degree of pungency decreases. Pungency can be quantitatively determined in onions by measuring enzymatically produced pyruvic acid. The onion industry quantifies a sweet onion as one having a...
Phytophthora ramorum is the causal agent of Sudden Oak Death (SOD), which is an economically and environmentally important disease causing up to 80% mortality of tanoaks in Pacific Northwest coastal forests (LeBoldus et al., 2022). There are currently four clonal lineages in the United States and Europe, named North America...
A broad range of factors influence wine grape quality and manipulation of these factors has stimulated interest among grape growers, wine makers, and research scientists alike. One such factor affecting wine grape quality is crop level, particularly for Pinot noir. Since the capacity of a vine to ripen fruit depends...
Extraction of natural gas from shale formations using the process of hydraulic fracturing (fracking) requires the use of thousands of cubic meters of fluid. Hydraulic fracturing fluids are pumped under pressure into shale formations, fracturing the shale and releasing pockets of trapped gases. When the pressure in a natural gas...
Grain protein concentration of wheat is influenced by numerous factors. When determining a wheat variety's ability to produce protein, the plant's ability to uptake and translocate nitrogen is critical. A greenhouse study was conducted to assess differences in nitrogen use among different wheat varieties. The objective was to determine if...
The rate of melanoma among Asian American has been steadily increasing in the past
five years, while in other Asian countries, such as Japan, the incident rate remains relatively the
same. Does the culture and societal attitudes toward skin cancer make a difference in the incident
rate of melanoma? This...
Cucurbits are grown in production agriculture in Oregon as well as in smallholder agriculture in tropical South America. Experimental research was performed at the OSU Research Farm in 2001 with direct-seeded cucurbits, and literature reviews were conducted to develop a characterization of smallholder farming in tropical South America. The two...
Natural products (secondary metabolites) are small organic molecules derived from plants, microbes, and marine organisms that have served as a diverse source of useful compounds pharmaceuticals. New biologically active natural products are critical as anti-infective and cancer drug leads. Cyanobacteria, or blue-green algae, produce diverse cytotoxic metabolites that represent potential...
New antibiotic compounds with novel mechanisms of action have become essential in response to the recent paradigm of increasingly resistant strains of bacteria. Secondary metabolites (natural products) from bacteria such as Actinobacteria serve as lead compounds for the development of pharmaceutical drugs widely used to fight bacterial, viral and fungal...
Polycyclic aromatic hydrocarbons (PAH) are common pollutants of aquatic systems in urban environments. Due to their lipophilicity, persistence, toxicity and carcinogenicity, they are chemicals of considerable concern. The Portland Harbor includes a large industrial section with numerous current and historic sources for PAH contamination. Runoff from a large metropolitan area...
Cell lines deficient in certain genes of the DNA mismatch repair (MMR) system show a marked resistance to the cell killing or apoptotic effects of DNA alkylating agents. The molecular mechanism for this resistance is not yet understood, but is currently of great interest because of the recent use of...
DNA mismatch repair (MMR) plays an important role in preserving genomic stability and reducing cancer risk. Environmental exposures to polycyclic aromatic hydrocarbons (PAHs) are believed to contribute significantly to carcinogenesis. PAHs are found in food, air, water and soil and upon bioactivation can form diol epoxides which are electrophilic in...
DNA mismatch repair (MMR) plays an important role in preserving genomic stability and reducing cancer risk. Environmental exposures to polycyclic aromatic hydrocarbons (PAHs) are believed to contribute significantly to carcinogenesis. PAHs are found in food, air, water and soil and upon bioactivation can form diol epoxides which are electrophilic in...
There are a number of problems associated with urban expansion and as cities continue
to grow the integration of green roofs can help to ease and offset many of them. This study
uses a combination of moss and Sedum as vegetation for experimental green roof sections in
order to test...
Nitrosomonas europaea is an autotrophic ammonia-oxidizer that has been found to cometabolize various halogenated hydrocarbons. This ability to cometabolize various compounds (many of which are considered contaminants) has caused N. europaea to be considered a potential bioremediational organism. However, ifN. europaea is to be used for bioremediation purposes, it will...
Recalcitrance to transformation makes genetic engineering of many valuable plants infeasible for practical use. Transitory host modification during transformation using transgenes offers a possible means of overcoming this obstacle. In prior work in our laboratory, the genes GA20ox7 and EBB1 were found to increase regeneration in vitro. In this project,...
Hart Mountain National Antelope Refuge (HMNAR) established in 1936, is 101,000 ha in area. The refuge was grazed by domestic livestock from the 1870s until their exclusion in 1990. Just prior to removal of livestock, photopoints were taken at various riparian and wetland locations throughout the refuge to document riparian...
Feeding cows flaxseed rich in polyunsaturated fatty acids has been shown to decrease the proportion of saturated fatty acids and increase the proportion of n-3 polyunsaturated fatty acids (PUFAs) in milk. Consuming products enriched in n-3 PUFAs have beneficial effects to human health; thus, feeding cows flaxseed could potentially increase...
Many studies in recent literature have shown a significant link between the abnormalities of a newly born individual and the type of environment that their mothers encountered during pregnancy. More specifically, over the last two decades, a discipline of research commonly known as “fetal programming” has linked abnormalities at birth...