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Filtering by: Committee Member Proebsting, William M. Non-Academic Affiliation Oregon State University. Bioresource Research

Method development for extraction and purification of dermal RNA from FVB/Nmice treated with environmental PAH mixtures

Creator:

Larkin, Andrew, Siddens, Lisbeth, and Krueger, Sharon

Abstract:

Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...

Resource Type:

Undergraduate Thesis and Presentation
Characterization of a Transgenic Mouse for Human Cytochrome P450 1B1 (CYP1B1)

Creator:

You, Hyekyoung Hannah

Abstract:

Cytochrome P450 1B1 (Cyp1B1) is a xenobiotic metabolizing enzyme, responsible for polycyclic aromatic hydrocarbon (PAH) oxidative metabolism. CYP1B1 metabolically activates several species of environmentally relevant procarcinogenic PAHs to reactive carcinogens. PAHs are aryl hydrocarbon receptor (AhR) agonists, leading to CYP1B1 upregulation. Though CYP1B1 is highly conserved across species, wild type...

Resource Type:

Honors College Thesis

Full Text:

Environmental and Molecular Toxicology/Linus Pauling Institute William M. Baird, Committee Member