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  • Identification of Grape Rootstocks and Varietal Clones by DNA Fingerprinting

    Creator:
    Bakalinsky, Alan
    Abstract:
    Correct identification of grapevines is of extreme importance to the expanding U.S. viticulture and wine industries. Several recent cases of misidentifications have occurred because the tools for accurate typing of plants are either inadequate or lacking. We have been applying one type of DNA-based procedure that has shown great promise...
    Resource Type:
    Technical Report
    Full Text:
    Varietal Clones by DNA Fingerprinting Alan Bakalinsky Department of Food Science and Technology

  • Gadusol Production in Saccharomyces cerevisiae

    Creator:
    Holzwarth, Garrett
    Abstract:
    Gadusol is a UV-B-absorbent compound found in fish and other marine organisms where it is presumed to play a role as a sunscreen and antioxidant. In light of commercial potential as a replacement for problematic synthetic sunscreens, a process to produce recombinant gadusol in the yeast Saccharomcyces cerevisiae was investigated....
    Resource Type:
    Masters Thesis
    Full Text:
    possibilities it offers. Alan Bakalinsky is a co-founder and co-owner of Gadusol Laboratories, a company that

  • An Approach to Increasing Yields of a Sunscreen Compound in Recombinant Yeast

    Creator:
    Vu, Van Anh
    Abstract:
    The yeast Saccharomyces cerevisiae, commonly used in winemaking, baking, and brewing, also serves as a host for the production of therapeutically valuable pharmaceuticals. Recently, the Bakalinsky laboratory constructed a recombinant yeast strain expressing two zebrafish (Danio rerio) genes in order to produce a UV-protective “sunscreen” compound called gadusol. An antioxidant...
    Resource Type:
    Honors College Thesis
    Full Text:
    . Abstract approved: ______________________________________________________ Alan Bakalinsky The

  • Nutritional and Genetic Aspects of Sulfite Excretion During Fermentation by Wine Strains of Saccharomyces cerevisiae

    Creator:
    Alessandri, María P.
    Abstract:
    Reductive sulfate assimilation, the biological process by which sulfur-containing amino acids and key derivatives are synthesized from sulfate, is broadly shared among bacteria, fungi, and plants. It is the major, if not sole source of methionine and cysteine for Saccharomyces cerevisiae during wine fermentation. Two obligate intermediates formed in the...
    Resource Type:
    Masters Thesis
    Full Text:
    : ______________________________________________________ Alan T. Bakalinsky Reductive sulfate assimilation, the biological process by which

  • Applied and basic aspects of sulfite metabolism in Saccharomyces cerevisiae

    Creator:
    Park, Hoon
    Abstract:
    In an effort to understand the basis for sulfite detoxification in S. cerevisiae, the functions of two genes were analyzed. SSU1, which encodes a plasma membrane protein, was found to be required for efficient sulfite efflux. FZFl-4, a dominant allele of a transcriptional activator of SSUl, was also found to...
    Resource Type:
    Dissertation
    Full Text:
    Aspects of Sulfite Metabolism in Saccharomyces cerevisiae. Abstract approved: Alan T. Bakalinsky

  • Genetic aspects of sulfite tolerance in Saccharomyces cerevisiae

    Creator:
    Avram, Dorina
    Resource Type:
    Dissertation
    Full Text:
    Abstract approved: Alan T. Bakalinsky Sulfite is a normal, but potentially toxic metabolite, in S

  • Bioethanol production from lignocellulosicbiomass: assessing genes linked to acetic acid stress in Saccharomyces cerevisiae

    Creator:
    Montgomery, Tyler P.
    Abstract:
    Lignocellulosic biomass represents a vast and renewable source of fermentable sugar for production of biofuels. However, native lignocellulose—comprised of cellulose, hemicellulose and lignin—is refractory to degradation because the crystalline cellulose is not easily hydrolyzed by cellulases. Standard chemical treatments of lignocellulose to reduce the crystallinity of cellulose prior to enzymatic...
    Resource Type:
    Honors College Thesis
    Full Text:
    : _________________________________________________ Alan T. Bakalinsky Lignocellulosic biomass represents a vast and renewable

  • In vivo detoxification of acetic acid in Saccharomyces cerevisiae

    Creator:
    Ding, Jun
    Abstract:
    The studies described in this thesis were motivated by ongoing efforts to develop lignocellulosic biomass as an efficient and practical source of renewable energy. Enormous problems complicate these efforts to reduce reliance on greenhouse gas-generating fossil fuels. Simply obtaining the fermentable sugars available in the cellulose and hemicellulose components of...
    Resource Type:
    Dissertation
    Full Text:
    cerevisiae Abstract approved: Alan T. Bakalinsky The studies described in this thesis

  • A study of antimutagenicity in yogurt

    Creator:
    Sudarshan, Nadathur R.
    Abstract:
    The purpose of this study was to identify antimutagens in yogurt active against the experimental colon carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Our initial experiments showed that acetone extracts of yogurt, or milk fermented by various lactic acid bacteria were antimutagenic against MNNG and 3,2'-dimethyl-4-aminobiphenyl (DMAB) in the Ames test (Salmonella typhimurium TA...
    Resource Type:
    Dissertation
    Full Text:
    Antimutagenicity in Yogurt Abstract Approved: ^ Alan T. Bakalinsky The purpose of this study was to identify

  • Study of sulfite mutants of Saccharomyces cerevisiae

    Creator:
    Wightman, JoLynne Dee
    Abstract:
    Sulfite mutants representing five complementation groups, previously derived from an ethyl methanesulfonate-treated haploid strain of Saccharomyces cerevisiae were studied. Although the wildtype S. cerevisiae strain used (isogenic to X2180-1 A) had a basal tolerance for sulfite (7 μM free H₂SO₃), the sensitive and resistant mutants were found to tolerate less...
    Resource Type:
    Masters Thesis
    Full Text:
    : A Study of Sulfite Mutants of Sacchammvces cerevisiae Abstract approved: Alan T. Bakalinsky