Nitrobacter hamburgensis X14 is a facultative lithoautotroph that conserves energy from the
oxidation of nitrite (NO
2 ) and fixes carbon dioxide (CO2) as its sole source of carbon. The
availability of the N. hamburgensis X14 genome sequence initiated a re-examination of its
mixotrophic and organotrophic potential, as genes encoding...
Although ammonia-oxidizing archaea (AOA) and bacteria (AOB) coexist in most non-acidic agricultural soils, the factors that influence their relative contributions to soil nitrification activity remain unclear. A 2- to 4-d whole soil microcosm assay was developed, utilizing the aliphatic C₈ alkyne 1-octyne to inactivate AOB-driven nitrification activity without impacting AOA...
The placement of ‘Pseudomonas butanovora’ in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA–DNA reassociation data are presented and the transfer of ‘P. butanovora’ to the genus Thauera is proposed. The original...
It is well known that the ratio of ammonia oxidizing archaea (AOA) and bacteria (AOB) ranges widely in soils, but no data exist on what might influence this ratio, its dynamism, or how changes in relative abundance influences the potential contributions of AOA and AOB to soil nitrification. By sampling...
Nitrosomonas europaea is a chemolithoautotrophic bacterium that oxidizes ammonia (NH₃) to obtain energy for growth on carbon dioxide (CO₂) and can also produce nitrous oxide (N₂O), a greenhouse gas. We interrogated the growth, physiological, and transcriptome responses of N. europaea to conditions of replete (>5.2 mM) and limited inorganic carbon...
Soluble butane monooxygenase (sBMO), a three-component di-iron monooxygenase complex
expressed by the C2–C9 alkane-utilizing bacterium Thauera butanivorans, was kinetically
characterized by measuring substrate specificities for C1–C5 alkanes and product inhibition
profiles. sBMO has high sequence homology with soluble methane monooxygenase (sMMO) and
shares a similar substrate range, including gaseous and...
To gain a better understanding of how rapidly microbial communities respond to different magnitudes of perturbation that mimic minor or catastrophic disturbances.
Two montane sites in the western Cascade Mountains of Oregon with adjacent areas of forest and meadow vegetation were studied. A reciprocal transplant experiment evaluated both minor (soil...
Ammonia (NH₃)-oxidizing bacteria (AOB) and thaumarchaea (AOA) co-occupy most soils, yet no short-term growth-independent method exists to determine their relative contributions to nitrification in situ. Microbial monooxygenases differ in their vulnerability to inactivation by aliphatic n-alkynes, and we found that NH₃ oxidation by the marine thaumarchaeon Nitrosopumilus maritimus was unaffected...
Climate change models predict that future precipitation patterns will entail lower-frequency but larger rainfall events, increasing the duration of dry soil conditions. Resulting shifts in microbial C cycling activity could affect soil C storage. Further, microbial response to rainfall events may be constrained by the physiological or nutrient limitation stress...
We report evidence for the simultaneous production of J/ψ and Υ mesons in 8.1 fb⁻¹ of data collected at s√=1.96 TeV by the D0 experiment at the Fermilab pp¯ Tevatron Collider. Events with these characteristics are expected to be produced predominantly by gluon-gluon interactions. In this analysis, we extract the effective cross...