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  • An Investigation into the Functional Properties of the Deafness Causing Protein Otoferlin

    Creator:
    Manchanda, Aayushi
    Abstract:
    Otoferlin is a C2-domain, transmembrane protein whose mutated form is responsible for the DFNB9 subtype of prelingual hearing impairment. Mutations in otoferlin cause an autosomal recessive, non-syndromic form of deafness that is characterized by auditory neuropathy/ auditory dissynchrony (AN/AD), meaning that the outer hair cells (OHCs) are normal, and the...
    Resource Type:
    Dissertation
    Full Text:
    place in my heart. I would like to thank my advisor Dr Colin P. Johnson for his continued support and

  • Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

    Creator:
    Codding, Sara Jone
    Abstract:
    The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
    Resource Type:
    Dissertation
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    :________________________________________________________ Colin P. Johnson The focus of this dissertation was to understand protein function

  • Initial in vitro and in vivo Characterization of the Membrane Trafficking Protein Fer1L6

    Creator:
    Wolk, Chelsea
    Abstract:
    A fundamental difference between prokaryotic and eukaryotic cells is the presence of membrane bound organelles in eukaryotes. The dynamics of membrane trafficking within the cell are responsible for everything from intercellular communication and cell homeostasis, to mitosis, cell migration, and differentiation. These processes require exocytosis and compensatory endocytosis, often working...
    Resource Type:
    Dissertation
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                Colin P. Johnson      Abstract  A fundamental difference between prokaryotic and eukaryotic cells is

  • Characterizing the Functional Properties of Otoferlin, Essential for Neurotransmission in Inner Hair Cells of the Cochlea

    Creator:
    Narayanappa, Murugesh
    Abstract:
    Hearing loss is one of the most common defect, affecting 360 million people worldwide due to several factors including congenital, present at or soon after birth or acquired with age. Congenital hearing loss affects 32 million children in the world. The economic impact of hearing loss is estimated to cost...
    Resource Type:
    Dissertation
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    . Abstract approved: Colin P. Johnson Hearing loss is one of the most common defect

  • Conformational Consequences: Calcium Regulates the Association Between Dysferlin C2A & PI(4,5)P2

    Creator:
    Otto, Shauna C.
    Abstract:
    Dysferlin is a ∼230 kDa terminally anchored membrane protein that is ubiquitously expressed, but is particularly enriched in skeletal and cardiac muscle tissue. Mutations covering the length of the protein have been linked to muscle wasting diseases including limb-girdle muscular dystrophy and Myoshi myopathy. Dysferlin has been shown to play...
    Resource Type:
    Dissertation
    Full Text:
    approved: Colin P. Johnson Dysferlin is a ∼230 kDa terminally anchored membrane protein that is ubiqui

  • Establishing Larval Zebrafish as an In Vivo Model Organism for Characterizing the Roles of Otoferlin, a Sensory Hair Cell Protein Essential for Hearing

    Creator:
    Chatterjee, Paroma
    Abstract:
    Auditory defects and disorders are prevalent at all ages and affect 8% of the population in developed nations including newborns and children. Congenital hearing loss is the most common birth defect and it is estimated that 1 in 1000 children are affected by deafness at birth or before the onset...
    Resource Type:
    Dissertation
    Full Text:
    : _________________________________________ Colin P. Johnson Abstract Auditory defects and

  • Single-molecule in vitro Characterization of Otoferlin

    Creator:
    Hams, Nicole J.
    Abstract:
    Congenital hearing loss is a common disorder and over 60 mutations in the sensory hair cell protein, otoferlin, have been linked to pre-lingual deafness. Although otoferlin is essential for hearing, the large size and low solubility of the protein have limited approaches to study its function on a molecular-level. To...
    Resource Type:
    Dissertation
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    of Otoferlin Abstract Approved: Colin P. Johnson Congenital

  • Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

    Creator:
    Perkins, Arden
    Abstract:
    Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
    Resource Type:
    Dissertation
    Full Text:
    : . P. Andrew Karplus Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide

  • The initial on-site supervision experiences of school counseling interns

    Creator:
    Ward, Colin C.
    Abstract:
    The purpose of this study was to investigate the initial on-site supervision experience of school counseling interns. Current counseling supervision research has failed to address dynamics specific to the context of school counselor training and professional development. This gap in the research suggest that examining the phenomenological experience of what...
    Resource Type:
    Dissertation
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    counseling interns AN ABSTRACT OF THE DISSERTATION OF Colin C. Ward for the degree of Doctor of Philosophy

  • Zero Kinetic Energy Photoelectron Spectroscopy of Polycyclic Aromatic Hydrocarbons

    Creator:
    Harthcock, Colin
    Abstract:
    In this dissertation, I describe spectroscopic studies of jet-cooled polycyclic aromatic hydrocarbons (PAH) and one nitrogen substituted PAH (PANH) using pulsed field zero kinetic energy (ZEKE) photoelectron spectroscopy and resonantly enhanced multiphoton ionization (REMPI) spectroscopy. Recently, there has been a demand for far-infrared (FIR) spectral information of astrophysically relevant molecules...
    Resource Type:
    Dissertation
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    Aromatic Hydrocarbons AN ABSTRACT OF THE DISSERTATION OF Colin Harthcock for the degree of Doctor