The objective of this study was to examine optimum conditions for primary
culture of porcine preadipocytes. Dorsal subcutaneous adipose tissue was obtained
from biopsies of 40-50 kg pigs and digested with collagenase in Krebs Ringer
Bicarbonate buffer. Isolated stromal vascular cells were cultured in different test media.
Stromal vascular cells...
In recent years, the general public has recognized the dangers of a high fat diet and
are demanding meat with lower fat content. This demand has stimulated research in the
growth and regulation of adipocytes. However, despite much effort, no adipocyte-specific
plasma membrane markers from any species are available as...