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  • Machovina CUE poster.pptx

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Full Text:
    . Engineering Bioorthogonal Reactions on Proteins in Live Cells Melodie M. Machovina and Ryan A. Mehl Labeling

  • Machovina CUE poster.pdf

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Full Text:
     Cells   Melodie  M.  Machovina  and  Ryan  A.  Mehl   Labeling  of  biomolecules

  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster

  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster

  • Exploring phylogeny of Rhodococcus fascians isolates using 16S ribosomal DNA sequence analysis

    Creator:
    Sandberg, Eva Song
    Abstract:
    Rhodococcus fascians is a Gram-positive, pleiomorphic actinomycete that causes leafy gall formation in a wide range of plant hosts. This particular phytopathogen has been a focus of research at the Oregon State University Plant Clinic, a diagnostic laboratory. Phylogeny of R. fascians isolates obtained from Plant Clinic submissions was determined...
    Resource Type:
    Honors College Thesis
    Full Text:
    Melodie Putnam Rhodococcus fascians is a Gram-positive, pleiomorphic actinomycete that causes leafy gall

  • The Identification of Ophiostoma novo-ulmi subsp. americana from Portland Elms

    Creator:
    Au, Benjamin
    Abstract:
    Dutch elm disease (DED) is a disease of elm trees caused by three species of Ascomycota fungi: Ophiostoma ulmi, Ophiostoma novo-ulmi, and Ophiostoma himal-ulmi. There are also two subspecies of O. novo-ulmi: subsp. americana and subsp. novo-ulmi. The pathogen is spread by bark beetles, which inhabit and traverse different elms....
    Resource Type:
    Honors College Thesis
    Full Text:
    : Melodie Putnam Dutch elm disease (DED) is a disease of elm trees caused by three species of

  • Characterizing the Plant-Associated Symbionts, Rhodococcus and Agrobacterium

    Creator:
    Fuller, Skylar L.
    Abstract:
    Symbioses are a spectrum of interactions between organisms living in closeassociation. These intimate interactions range from mutualism, in which bothorganisms benefit, to parasitism, where one organism benefits at the expense ofthe other. Horizontal gene transfer is the acquisition of genes independent ofvertical transmission and demonstrably promotes the transition of bacteria...
    Resource Type:
    Masters Thesis
    Full Text:
    ., Amasino, R. M., Nester, E. W., and Gordon, M. P. 1984. T-DNA of Agrobacterium tumefaciens encodes an

  • Viticulture & Enology Technical Newsletter : November 2012

    Creator:
    Oregon Wine Research Institute
    Abstract:
    This issue is packed with new information from various research trials conducted here in Oregon, whether it is from vineyard plots, the wine lab, or the growth chamber, results are likely to have impact on vineyard and winery production here in Oregon. James Osborne, Enology Extension Specialist, opens this month’s...
    Resource Type:
    Other
    Full Text:
    notice this month is the invited article by Melodie Putnam, director of the OSU Plant Clinic. Her

  • Landscape Genomics and Climatic Responses of Willamette Valley Alders

    Creator:
    Kral, George L.
    Abstract:
    The climate of the Pacific Northwest is in flux, and existing forest ecosystems are stressed and poised to shift in fundamental ways, with or without human intervention. This dissertation probes the nature of forest responses to environmental change through investigations of morphology and genetics of three species of alder co-occurring...
    Resource Type:
    Dissertation
    Full Text:
    , Data curation, Formal analysis. Melodie L Putnam: Formal analysis, Investigation, Methodology

  • Hop cone tip blight : a new disease in the Pacific Northwest

    Creator:
    Bienapfl, John C.
    Abstract:
    A necrosis at the tip of cones was observed on hop (Humulus lupulus), cultivar "Nugget", grown in Oregon in the early 1990's. Fusarium sambucinum and F. avenaceum were recovered from symptomatic cones in 1998 and preliminary inoculation experiments suggested both Fusarium species could cause hop cone necrosis. Studies were carried...
    Resource Type:
    Masters Thesis
    Full Text:
    Blight: A New Disease in the Pacific Northwest Abstract approved: Cynthia M. Ocamb A necrosis at the