Index Catalog // ScholarsArchive@OSU

Creator:: Machovina, Melodie M. and Mehl, Ryan A.
Abstract:: This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:: . Engineering Bioorthogonal Reactions on Proteins in Live Cells Melodie M. Machovina and Ryan A. Mehl Labeling

Creator:: Machovina, Melodie M. and Mehl, Ryan A.
Abstract:: This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:: Cells Melodie M. Machovina and Ryan A. Mehl Labeling of biomolecules

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis, Edward W., II, Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
Resource Type:: Article
Full Text:: Pathogenic Rhodococcus Reveals Genetic Novelties in Virulence Loci Allison L. Creason1,2., Olivier M

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis, Edward W., II, Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
Resource Type:: Other

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis II, Edward W., Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis II, Edward W., Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis, Edward W., II, Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
Resource Type:: Article

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis II, Edward W., Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: We report the sequence and characterization of the genomes of 21 isolates of Rhodococcus. Next generation sequencing technology was used to generate genome sequences. The reads for each genome were de novo assembled. The contigs were re-ordered, using the genome sequence of A44a as a reference. The program, Prokka, was...
Resource Type:: Dataset

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