Methods have been developed to measure the time required to
complete replication of an adenovirus DNA molecule and the time
between rounds of replication of the viral genomes. These methods
show that 22 minutes are required to complete replication of an
adenovirus DNA molecule at 37° C and that this...
Replication of plasmid molecules containing a single
adenovirus terminal sequence and specially arranged
inverted repeat sequences were studied. When the plasmids
were linearized to expose the adenovirus terminal sequence
and transfected into 293 cells with helper adenovirus DNA,
a larger molecule was detected. The new molecule
contained adenovirus terminal sequences...
Adenovirus initiates strand-displacement DNA replication from
origins located in identical inverted terminal repetitions (ITRs).
Panhandle structures, formed by base-pairing between ITRs on the
displaced strands, have been proposed as replication intermediates for
complementary strand synthesis. Plasmid molecules containing a single
adenovirus terminal sequence and specially arranged inverted repeat
sequences were...
Replication of plasmid molecules containing either a left-end or a
right-end adenovirus origin was investigated. When the plasmids were
linearized to expose their respective adenovirus termini, mixed, and
transfected with helper adenovirus DNA into the human 293 cell line, a
new molecule was detected. The new molecule was the size...
The adenovirus replication origins reside in the inverted terminal
repetition (ITR) sequences. Replication proceeds unidirectionally by a
strand-displacement mechanism from either or both origins to produce
duplex daughter DNA plus a displaced parental strand. The displaced
strand can be duplicated by two different routes. The cis pathway involves
intramolecular hybridization...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...