Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
Wine grape pomace (WGP), the byproduct from winemaking, is a good source of polyphenols and dietary fibers, and may be utilized as antioxidant dietary fibers (ADF) for food applications. The objectives of this thesis research were to first determine the phenolic compounds, antioxidant and antimicrobial activities in red WGP under...
Despite the advances in surgery, physical therapy, and pharmaceutical agents, muscle
dysfunction (i.e., atrophy and weakness) continues to impair recovery from an anterior
cruciate ligament (ACL) injury and surgery. Ischemia-reperfusion injury during surgery
and the subsequent limb disuse are two events experienced by patients having ACL
surgery. Oxidative stress and...
Shrimp waste samples were extracted with a variety of organic solvents. Each fraction was measured for antioxidant activity by determining the rate of oxidation of β-carotene-linoleic acid in an emulsion system. An ethanol extract exhibited the highest antioxidant activity. Purification of the most active fraction was accomplished by thin layer...
Blackberry (Rubus sp.) fruit are a good source of phenolic compounds and anthocyanins, and are consumed in fresh and processed forms. Though organic products become more popular, limited information is available about how different organic production methods affect the post-harvest quality of blackberry fruit. The objective of this project was...
The effect of increased manganese superoxide dismutase (Mn-SOD) and cytosolic ascorbate peroxidase (cAPX) on various abiotic stresses was studied using transgenic tomato (Lycopersicon esculentum) plants. Transformants were selected in vitro based on kanamycin resistance, confirmed by PCR and northern blot analyses. Northern blots showed an enhanced gene expression of Mn-SOD...
β-chitin/chitosan extracted from newly utilized jumbo squid (Dosidicus gigas) pens were investigated in respect to their physicochemical properties, polymeric structures, deacetylation and depolymerization characteristics, and antioxidant and antibacterial activities. These functional properties were further compared with α-chitin/chitosan prepared from shrimp shells. Due to the low mineral content (< 1%) and...