Techniques were developed for extracting histones from isolated
chromatin of encysted embryos of Artemia salina. Histone extracts
from embryos collected at eight time intervals after rehydration and
initiation of development were analyzed by electrophoresis for qualitative
and quantitative changes in composition. Histone to DNA ratios
were determined for the same...
The cytoplasmic isozyme of malate dehydrogenase (s-MDH) has been isolated from brine shrimp nauplii to a state of high purity with a protocol involving ammonium sulfate fractionation and chromatography on DEAE-cellulose, followed by sequential passes over cellulose phosphate columns at pH 5.8 then 5.0. The purified enzyme is free of...