Primary cultures of stromal-vascular (S-V) cells from adipose tissue were
used to investigate the regulation of preadipocyte development. Differentiation of
S-V cells was found to be under hormonal control. Insulin and glucocorticoids are
essential for S-V cell differentiation in culture.
S-V cells from both newborn and mature pig adipose tissue...
Mouse embryo cells cultured in vitro in serum-supplemented media undergo growth
crisis, resulting in the loss of genomically normal cells prior to the appearance of
established, aneuploid cell lines. I used the technique of serum-free cell culture to develop
a serum-free mouse embryo (SFME) cell line in which serum was...
The profile of the inner-tubing gas pressure for a tubular membrane aeration system was quantified. The correlations among the overall volumetric oxygen transfer coefficient (k[subscript L]a), the inner-tubing pressure, the tubing tightness, and the gas throughput are experimentally analyzed. A mathematical model was developed to describe the underlying phenomena. The...