Analytical chemistry is an area of chemistry primarily focused on the study and use of instruments for separation, identification, and quantification of an analyte of interest. Specifically, separation science within analytical chemistry often refers to the process of dividing mixtures into their small component parts based on differences in their...
An all-glass gas chromatograph was developed for the analysis
of corrosive metal halides. Included in the apparatus is a new liquid
sampling system designed for fast repetitive sampling of corrosive
liquids in an inert atmosphere. A flame photometer detector was developed
to permit the detection of corrosive solutes in the...
A new general method is developed for the optimization of
HPLC ternary or pseudo-quaternary mobile phases which are
represented by the trilinear coordinate system. This method can
predict the global optimum of the mobile phase composition. The
global optimum composition along each edge of the triangle and
the corresponding selectivity...
Gradient elution chromatography is frequently used for the preparative separation
of peptides and proteins. Separations at high loadings are often avoided because peaks
become asymmetrical and saturate the detector. However, non-linear interactions which
become important at high loadings may actually improve the separation with greater
concentrations of the product being...
An analytical technique has been developed for the determination of methylmercury compounds in environmental samples. The technique is based on a two-stage procedure. In the first stage, methylmercury compounds are isolated from the sample matrix as methylmercury chloride (MeRgCl) by distillation. The distillation procedure is based on a series of...
Optimization of preparative nonlinear chromatography was carried out for
the first time for a biomolecule mixture. Conventional wisdom on optimization,
which roots from analytical chromatography, dictates optimizing resolution in an
analytical column and obtaining similar separation in a large column for isolation.
Such a method of optimization significantly under uses...
Ion exchange chromatography methods which have been
developed in recent years appear to offer a sensitive technique that
can be utilized very advantageously in studies on various proteins
and their properties. The application of such a procedure for the
successful fractionation of bovine sarcoplasmic proteins should stimulate
interest and research...