A sensitive assay for the analysis of contaminating segments
in pseudorecombinants constructed from a seed-transmissible (CVM-Pg)
and a non-seed-transmissible (CMV-Le) strain of cucumber mosaic
virus was developed using partial RNase T₁ digestion. Isolated
genomic RNA segments produced unique digestion patterns when 3'-
end labeled, treated with RNase T₁, and subjected...
The frequency of aphid transmission of cucumber mosaic virus
from diseased plants at different times after inoculation was determined.
A cycle of infectious virus was found. Primary leaves
of lima bean plants were inoculated mechanically when the first trifoliate
leaf appeared and were kept at different temperatures. At
24° C...
A survey of major bean (Phaseolus vulgaris) breeding programs and
seed producing areas in Idaho, Washington, and Oregon was conducted to
determine the occurrence of cucumber mosaic virus (CMV), recently reported
to be seedborne in beans. Some 259 plants, sampled from breeding
lines of diverse germplasm, were found by enzyme-linked...