Calcium activated proteases, or calpains, are activated in cardiac muscles under conditions of ischemia/reperfusion and hypoxia. Their activity in skeletal muscle under similar conditions is poorly understood. We tested the hypothesis that hypoxia elevates calpain-mediated proteolysis in isolated glycolytic (extensor digitorum longus, EDL) and oxidative (soleus, SOL) mouse muscles studied...
We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain...
Cell motility and invasion are important for development, immunity, wound healing, and tumor cell metastasis. Cells on two dimensional substrates migrate in three steps: protrusion of the front end, translocation of the cell body, and retraction of the rear end. For cells to migrate efficiently, these steps need to be...